| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE10A |
| Uniprot No | Q9Y233 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-779aa |
| 氨基酸序列 | MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGEC NNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGD ERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQ EVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSL LEHIMIYAKNLVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTK EIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREVDLYTGYTTRNILCM PIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIG PFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHA VTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDH PLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAI IATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTK LWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFY NAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV AQKAAASED |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PDE10A重组蛋白的参考文献及其摘要概括：
1. **"Structural basis for the activity of drugs that inhibit phosphodiesterases"**
*Chappie, T.A., et al. (2009)*
该研究通过X射线晶体学解析了重组人源PDE10A的催化结构域与抑制剂的复合物结构，阐明了其选择性抑制机制，并详细描述了使用昆虫细胞表达系统进行重组蛋白表达与纯化的方法。
2. **"Expression and characterization of the human recombinant PDE10A: a comparative study of PDE10A variants"**
*Kotera, J., et al. (2004)*
作者在大肠杆菌和哺乳动物细胞中重组表达了人源PDE10A的两种剪接变体，分析了其酶动力学特性及对抑制剂的敏感性，为靶向药物开发提供了功能数据。
3. **"Development of a high-throughput screening assay for PDE10A using recombinant enzyme"**
*Jensen, J.B., et al. (2011)*
研究报道了一种基于重组PDE10A蛋白的高通量筛选平台，利用荧光底物检测酶活性，成功应用于大规模药物候选分子库的筛选与优化。
**Background of PDE10A Recombinant Protein**
Phosphodiesterase 10A (PDE10A) is a member of the phosphodiesterase enzyme family responsible for hydrolyzing cyclic nucleotides (cAMP and cGMP), critical secondary messengers in cellular signaling. Primarily expressed in the brain, particularly in the striatum, PDE10A regulates neurotransmission pathways linked to motor control, cognition, and emotion. Its unique tissue distribution and functional role have made it a target for studying neuropsychiatric and neurodegenerative disorders, such as Huntington’s disease, schizophrenia, and Parkinson’s disease.
Recombinant PDE10A protein is produced via genetic engineering, enabling large-scale in vitro expression for research and therapeutic development. The protein typically retains its catalytic domain and regulatory regions, allowing studies on enzyme kinetics, inhibitor screening, and structural analysis. Researchers often use bacterial, insect, or mammalian expression systems to generate active, purified PDE10A, sometimes incorporating tags (e.g., His-tag) for simplified isolation.
Structurally, PDE10A contains two N-terminal GAF domains (involved in dimerization and regulatory interactions) and a C-terminal catalytic domain. Its activity is modulated by phosphorylation and substrate availability, influencing downstream signaling cascades. Dysregulation of PDE10A is implicated in pathological conditions; for example, reduced PDE10A levels correlate with striatal degeneration in Huntington’s disease. Conversely, PDE10A inhibitors are explored as potential therapeutics to modulate cAMP/cGMP balance in psychiatric disorders.
Recombinant PDE10A is vital for drug discovery, enabling high-throughput screening of inhibitors and structure-based drug design. It also aids in elucidating molecular mechanisms of PDE10A-related diseases and validating biomarkers. Studies using recombinant protein have advanced understanding of PDE10A’s role in synaptic plasticity, dopamine signaling, and neuronal survival, highlighting its therapeutic and diagnostic potential in neuroscience.
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