| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRABP1 |
| Uniprot No | P29762 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-137aa |
| 氨基酸序列 | PNFAGTWKMRSSENFDELLKALGVNAMLRKVAVAAASKPHVEIRQDGDQFYIKTSTTVRTTEINFKVGEGFEEETVDGRKCRSLPTWENENKIHCTQTLLEGDGPKTYWTRELANDELILTFGADDVVCTRIYVRE |
| 预测分子量 | 42.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CRABP1重组蛋白的代表性文献摘要(内容基于公开研究主题概括，非真实文献)：
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1. **文献名称**: *"Expression and Purification of Recombinant CRABP1 for Structural Studies"*
**作者**: Zhang Y. et al.
**摘要**: 报道了利用大肠杆菌系统高效表达并纯化重组人源CRABP1蛋白的方案，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证其与视黄酸(RA)的特异性结合活性，为后续结构解析奠定基础。
2. **文献名称**: *"Crystal Structure of CRABP1 Reveals Retinoid Binding Mechanism"*
**作者**: Kleywegt G.J. & Jones T.A.
**摘要**: 通过X射线晶体学解析了重组CRABP1与全反式视黄酸的复合物结构，揭示了β-桶状结构中关键氨基酸残基如何通过氢键和疏水作用固定配体，阐明了其转运RA的分子机制。
3. **文献名称**: *"CRABP1 Modulates Retinoic Acid Signaling in Neural Differentiation"*
**作者**: Napoli J.L. et al.
**摘要**: 利用重组CRABP1蛋白进行体外细胞实验，发现其通过调控RA的胞内浓度影响神经干细胞分化，证实CRABP1在RA信号通路中起“缓冲剂”作用，平衡RA的代谢与信号传递。
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**提示**：如需真实文献，建议在PubMed或Web of Science检索关键词“CRABP1 recombinant protein”或结合具体研究方向(如结构、功能、疾病关联)筛选近年论文。
**Background of CRABP1 Recombinant Protein**
Cellular retinoic acid-binding protein 1 (CRABP1) is a member of the intracellular lipid-binding protein family, primarily involved in regulating the transport, metabolism, and signaling of retinoic acid (RA), an active metabolite of vitamin A. CRABP1 binds RA with high affinity, facilitating its intracellular trafficking and modulating its availability for nuclear receptors like retinoic acid receptors (RARs), which regulate gene expression critical for development, differentiation, and homeostasis.
CRABP1 is distinct from its isoform CRABP2 in tissue distribution and functional roles. While CRABP2 is associated with delivering RA to RARs for transcriptional activation, CRABP1 is enriched in tissues such as the nervous system, muscle, and skin, where it may buffer RA levels or direct RA toward metabolic pathways (e.g., cytochrome P450-mediated degradation). Structural studies reveal a conserved β-barrel fold with a ligand-binding pocket that accommodates RA, stabilized by specific hydrophobic and polar interactions.
Recombinant CRABP1 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. Its production enables detailed biochemical and biophysical studies, including ligand-binding assays, structural analysis, and interaction mapping with RA or downstream targets. Researchers use CRABP1 recombinant protein to investigate RA signaling dysregulation in diseases such as cancer, neurodegeneration, and developmental disorders. For example, altered CRABP1 expression has been linked to neuroblastoma progression and impaired neural differentiation.
Overall, CRABP1 recombinant protein serves as a vital tool for dissecting RA-mediated pathways and developing therapeutic strategies targeting retinoid signaling.
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