| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSP1 |
| Uniprot No | P33241 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-339aa |
| 氨基酸序列 | MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPSEAPELDEDEGFGDWSQRPEQRQQHEGAQGTLDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSLSKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP |
| 预测分子量 | 64.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LSP1重组蛋白的虚构参考文献示例，基于合理推测的研究方向整理：
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1. **文献名称**：*LSP1 Recombinant Protein Modulates T-cell Signaling through Actin Cytoskeleton Remodeling*
**作者**：Chen, L., et al.
**摘要**：该研究通过大肠杆菌系统表达并纯化了功能性LSP1重组蛋白，发现其通过结合F-actin调控T细胞受体(TCR)下游信号传导，揭示了LSP1在免疫突触形成中的动态作用机制。
2. **文献名称**：*Structural Characterization of Recombinant LSP1 and Its Interaction with Inflammatory Chemokines*
**作者**：Martinez, R., & Park, J. H.
**摘要**：利用X射线晶体学解析了重组人源LSP1蛋白的N端结构域，证明其与CXCL12等趋化因子特异性结合，暗示LSP1可能通过调控炎症因子募集参与自身免疫性疾病病理过程。
3. **文献名称**：*LSP1 Knockdown and Recombinant Rescue in Breast Cancer Cell Migration*
**作者**：Gupta, S., et al.
**摘要**：研究发现乳腺癌细胞中LSP1表达缺失导致迁移增强，外源性添加重组LSP1蛋白可部分逆转该表型，提示LSP1作为肿瘤转移抑制因子的潜在治疗价值。
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**注**：以上文献为模拟示例，实际研究中请以真实发表的论文为准。如需查找真实文献，建议通过PubMed或Web of Science等数据库检索关键词“LSP1 recombinant protein”或“LSP1 protein function”。
LSP1 (Lymphocyte-Specific Protein 1), also known as SPTRL1 or WP34. is a cytoskeletal-associated protein predominantly expressed in hematopoietic cells, including lymphocytes, neutrophils, and macrophages. It belongs to the leukocyte-specific gene (LSG) family and plays a critical role in regulating immune cell motility, adhesion, and signal transduction. Structurally, LSP1 contains multiple functional domains, such as an N-terminal acidic region, a central proline-rich motif, and a C-terminal basic region, which facilitate interactions with actin filaments, signaling molecules (e.g., SH3 domain-containing proteins), and membrane components.
Recombinant LSP1 proteins are engineered using molecular cloning techniques to express and purify the protein in heterologous host systems (e.g., E. coli or mammalian cell lines). These recombinant variants often include affinity tags (e.g., His-tag) for simplified purification and detection. Researchers utilize recombinant LSP1 to investigate its molecular mechanisms in immune responses, particularly its role in modulating cytoskeletal dynamics during cell migration and its involvement in inflammatory pathways. Studies have linked LSP1 dysregulation to pathologies such as chronic inflammation, autoimmune diseases, and cancer metastasis, making it a potential therapeutic target.
The development of recombinant LSP1 has enabled advanced biochemical assays, structural studies, and drug screening. For example, it aids in mapping binding partners, characterizing post-translational modifications, and validating inhibitors targeting LSP1-mediated pathways. Its application extends to disease models, where recombinant proteins help elucidate LSP1's contribution to immune evasion in tumors or neutrophil dysfunction in sepsis. Overall, recombinant LSP1 serves as a vital tool for decoding immune cell biology and exploring therapeutic interventions in immune-related disorders.
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