| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCP1 |
| Uniprot No | P13796 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-627aa |
| 氨基酸序列 | ARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYHLLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVTATDVVRGNPKLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLYSDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVELGKNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRRYTLNILEEIGGGQKVNDDIIVNWVNETLREAKKSSSISSFKDPKISTSLPVLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYALPEDLVEVNPKMVMTVFACLMGKGMKRV |
| 预测分子量 | 72.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于LCP1重组蛋白的参考文献示例，实际文献需通过学术数据库进一步检索确认：
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1. **文献名称**: *Functional Characterization of Recombinant LCP1 in T-cell Motility*
**作者**: Zhang Y, et al. (2018)
**摘要**: 通过原核系统表达并纯化重组人LCP1蛋白，研究证实其通过调控肌动蛋白聚合促进T细胞迁移，为免疫细胞运动的分子机制提供新见解。
2. **文献名称**: *Structural Insights into LCP1-Actin Interaction Using Recombinant Protein Technology*
**作者**: Smith JR, et al. (2015)
**摘要**: 利用重组LCP1蛋白解析其与F-肌动蛋白结合的晶体结构，揭示了钙离子依赖性结合的关键结构域，阐明了细胞骨架动态调节的分子基础。
3. **文献名称**: *Recombinant LCP1 Enhances Metastatic Potential in Breast Cancer Cells*
**作者**: Lee H, et al. (2020)
**摘要**: 体外实验表明，重组LCP1过表达显著增加乳腺癌细胞的侵袭能力，提示其作为肿瘤转移标志物或治疗靶点的可能性。
4. **文献名称**: *High-Yield Purification of Recombinant LCP1 and Its Role in Macrophage Activation*
**作者**: Garcia M, et al. (2019)
**摘要**: 开发新型重组LCP1蛋白纯化方案，并证明其通过NF-κB通路调节巨噬细胞炎症反应，为自身免疫疾病研究提供工具。
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**提示**：以上内容为模拟生成，实际文献建议通过PubMed、Web of Science等平台以关键词“LCP1 recombinant protein”“L-plastin expression”等检索。
LCP1 (Lymphocyte Cytosolic Protein 1), also known as L-Plastin, is a member of the plastin family of actin-binding proteins. It plays a critical role in cytoskeletal reorganization, cell motility, and immune response regulation. Structurally, LCP1 contains two conserved actin-binding domains (ABDs) and a calcium-binding regulatory domain. Its activity is modulated by post-translational modifications, particularly phosphorylation, which influences its interaction with actin filaments.
Originally identified in hematopoietic cells, LCP1 is highly expressed in immune cells such as T cells, B cells, and macrophages. It facilitates immune cell functions like chemotaxis, adhesion, and antigen presentation by stabilizing dynamic actin networks. Dysregulation of LCP1 has been implicated in pathological conditions, including cancer metastasis, autoimmune disorders, and chronic inflammation. For example, elevated LCP1 levels in certain cancers correlate with enhanced tumor cell invasion and resistance to therapy.
Recombinant LCP1 protein is produced using expression systems like *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. This engineered protein retains functional domains essential for studying actin-binding mechanisms, immune signaling pathways, and drug screening. Researchers utilize it in *in vitro* assays, structural studies (e.g., X-ray crystallography), and disease models to explore its role in cellular dynamics or therapeutic targeting.
Commercial LCP1 recombinant proteins often include tags (e.g., His-tag, GST) for purification and detection. Quality control involves SDS-PAGE, Western blotting, and functional validation through actin polymerization assays. Its applications span basic research in cell biology, immunology, and translational studies aiming to develop inhibitors for cancer or inflammatory diseases.
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