| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRK2 |
| Uniprot No | Q5S007 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 968-2527aa |
| 氨基酸序列 | MRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLE LHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSR NDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGN KISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAA MPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHW KSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCL ENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKD IIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSA TVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLA VYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMS KITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQL VVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDEN ELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEG CPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYL LVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISP YMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITV PSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFN DGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRN IMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLL RQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLT RTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAD YGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDIL TTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKE NPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASI WLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVS GTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGT ADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWG GCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAK QNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTL CLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLK NVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKEL AEKMRRTSVE |
| 预测分子量 | 210 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRRK2重组蛋白的3篇代表性文献及其摘要概括：
1. **"LRRK2 kinase activity regulates autophosphorylation at serine 1292"**
- **作者**: Deng X et al. (Biochem J, 2014)
- **摘要**: 通过重组人源LRRK2蛋白的体外激酶实验，揭示了其自磷酸化位点Ser1292的活性依赖性调节机制，证明该位点可作为LRRK2激酶活性的生物标志物。
2. **"Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation"**
- **作者**: Steger M et al. (EMBO J, 2017)
- **摘要**: 利用重组LRRK2蛋白进行体外磷酸化筛选，鉴定出多种Rab GTPases(如Rab8A、Rab10)为LRRK2的直接底物，揭示了其在囊泡运输中的关键作用。
3. **"LRRK2 inhibitor screening using recombinant protein kinase domains"**
- **作者**: Ramsden N et al. (J Pharmacol Exp Ther, 2011)
- **摘要**: 基于重组LRRK2激酶结构域开发高通量抑制剂筛选平台，验证了多种ATP竞争性抑制剂对病理性突变体(如G2019S)的选择性抑制作用。
如需更多文献或特定研究方向，可进一步说明需求。
Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain protein belonging to the ROCO family, characterized by a unique combination of enzymatic and scaffolding domains. It contains a kinase domain, a GTPase domain (Ras-of-complex, ROC), and multiple protein-protein interaction domains, including leucine-rich repeats (LRR), a COR (C-terminal of ROC) domain, and a WD40 domain. LRRK2 is expressed in various tissues, with high levels observed in the brain, particularly in neurons and glial cells. It plays roles in membrane trafficking, autophagy, and cytoskeletal dynamics, though its precise physiological functions remain under investigation.
LRRK2 gained prominence due to its association with Parkinson’s disease (PD). Mutations in the LRRK2 gene, particularly the G2019S variant in the kinase domain, are the most common genetic cause of familial and sporadic PD. These mutations often enhance LRRK2 kinase activity, leading to neurotoxicity through mechanisms involving mitochondrial dysfunction, synaptic impairment, and α-synuclein aggregation. Studying LRRK2’s structure-function relationships and pathogenic mechanisms is critical for understanding PD pathophysiology.
Recombinant LRRK2 proteins, produced via expression systems like insect or mammalian cells, enable biochemical and structural studies. These proteins retain post-translational modifications and functional domains, allowing researchers to investigate kinase/GTPase activities, substrate interactions, and the effects of PD-linked mutations. Recombinant LRRK2 is widely used to screen kinase inhibitors, dissect signaling pathways, and develop biomarkers. Challenges include its large size (~280 kDa), autophosphorylation, and dynamic domain rearrangements. Despite these hurdles, recombinant LRRK2 remains a key tool for advancing therapeutic strategies targeting PD and related disorders.
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