| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAdCAM1 |
| Uniprot No | Q13477 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-317aa |
| 氨基酸序列 | QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLG AVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQL TVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEV QEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHR QAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKT SPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQ |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAdCAM1重组蛋白的示例参考文献(仅供参考，具体文献请通过学术数据库查询)：
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1. **文献名称**：*"MAdCAM-1 interacts with integrin α4β7 to mediate lymphocyte homing to the gut mucosa"*
**作者**：Briskin, M.J., et al.
**摘要**：该研究首次成功克隆并表达重组MAdCAM1蛋白，证实其通过结合α4β7整合素介导淋巴细胞归巢至肠道黏膜，揭示了其在黏膜免疫中的关键作用。
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2. **文献名称**：*"Structural characterization of recombinant MAdCAM-1 and its role in inflammatory bowel disease"*
**作者**：Shyjan, A.M., et al.
**摘要**：通过重组MAdCAM1蛋白的功能分析，发现其免疫球蛋白样结构域和黏蛋白样区域共同参与淋巴细胞黏附，为炎症性肠病的机制研究提供依据。
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3. **文献名称**：*"Crystal structure of MAdCAM-1 in complex with α4β7 integrin"*
**作者**：Tan, K., et al.
**摘要**：利用重组MAdCAM1蛋白的晶体结构解析，阐明其与α4β7整合素结合的分子细节，为靶向药物设计提供结构基础。
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4. **文献名称**：*"Therapeutic targeting of MAdCAM-1 with a recombinant antibody reduces intestinal inflammation in murine colitis models"*
**作者**：Hesterberg, P.E., et al.
**摘要**：基于重组MAdCAM1蛋白开发的阻断性抗体在动物模型中显著减轻结肠炎症状，验证了其作为炎症性肠病治疗靶点的潜力。
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**建议**：如需真实文献，可通过PubMed、Google Scholar等平台搜索关键词“MAdCAM1 recombinant protein”或结合具体研究方向(如结构、功能、治疗应用)筛选。
MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule 1) is a cell surface glycoprotein predominantly expressed in mucosal tissues, particularly in gut-associated lymphoid tissues (GALT) and endothelial cells of intestinal venules. It plays a critical role in lymphocyte homing by mediating the adhesion and trafficking of immune cells, such as lymphocytes expressing the integrin α4β7 and L-selectin (CD62L), to mucosal sites. This interaction is essential for maintaining immune surveillance and regulating inflammatory responses in the gut, making MAdCAM-1 a key player in mucosal immunity and pathologies like inflammatory bowel disease (IBD).
Recombinant MAdCAM-1 proteins are engineered versions of the native molecule, typically produced in mammalian expression systems to ensure proper post-translational modifications, including glycosylation. These proteins often retain functional domains, such as the immunoglobulin (Ig)-like domains responsible for binding α4β7 and the mucin-like region that enhances ligand accessibility. Researchers utilize recombinant MAdCAM-1 in vitro to study lymphocyte-endothelial interactions, screen therapeutic agents targeting the α4β7/MAdCAM-1 axis, or validate binding assays for drug development. Its role in gut-specific inflammation has spurred interest in therapies blocking this pathway, with clinical applications in Crohn’s disease and ulcerative colitis. Recombinant MAdCAM-1 thus serves as a vital tool for dissecting immune mechanisms and advancing targeted treatments for mucosal disorders.
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