| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOX5 |
| Uniprot No | Q96PH1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-765aa |
| 氨基酸序列 | MNTSGDPAQTGPEGCRGTMSAEEDARWLRWVTQQFKTIAGEDGEISLQEFKAALHVKESF FAERFFALFDSDRSGTITLQELQEALTLLIHGSPMDKLKFLFQVYDIDVCARQGASAGTE WGAGAGPHWASSPLGTGSGSIDPDELRTVLQSCLRESAISLPDEKLDQLTLALFESADAD GNGAITFEELRDELQRFPGVMENLTISAAHWLTAPAPRPRPRRPRQLTRAYWHNHRSQLF CLATYAGLHVLLFGLAASAHRDLGASVMVAKGCGQCLNFDCSFIAVLMLRRCLTWLRATW LAQVLPLDQNIQFHQLMGYVVVGLSLVHTVAHTVNFVLQAQAEASPFQFWELLLTTRPGI GWVHGSASPTGVALLLLLLLMFICSSSCIRRSGHFEVFYWTHLSYLLVWLLLIFHGPNFW KWLLVPGILFFLEKAIGLAVSRMAAVCIMEVNLLPSKVTHLLIKRPPFFHYRPGDYLYLN IPTIARYEWHPFTISSAPEQKDTIWLHIRSQGQWTNRLYESFKASDPLGRGSKRLSRSVT MRKSQRSSKGSEILLEKHKFCNIKCYIDGPYGTPTRRIFASEHAVLIGAGIGITPFASIL QSIMYRHQKRKHTCPSCQHSWIEGVQDNMKLHKVDFIWINRDQRSFEWFVSLLTKLEMDQ AEEAQYGRFLELHMYMTSALGKNDMKAIGLQMALDLLANKEKKDSITGLQTRTQPGRPDW SKVFQKVAAEKKGKVQVFFCGSPALAKVLKGHCEKFGFRFFQENF |
| 预测分子量 | 86,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOX5重组蛋白的3篇代表性文献及其摘要内容：
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1. **文献名称**：*Characterization of recombinant NADPH oxidase 5 (NOX5) protein in endothelial cells*
**作者**：S. BelAiba et al.
**摘要**：研究通过哺乳动物表达系统成功重组了人源NOX5蛋白，发现其钙离子依赖性活性显著影响内皮细胞的氧化应激反应，揭示了NOX5在血管功能调控中的潜在作用。
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2. **文献名称**：*Structural insights into the regulation of NOX5 by calcium and phosphorylation*
**作者**：K. Jagnandan et al.
**摘要**：通过重组NOX5蛋白的结构分析，阐明了钙结合域和磷酸化位点如何协同调节酶活性，为靶向NOX5的药物设计提供了分子基础。
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3. **文献名称**：*Recombinant NOX5 expression in HEK293 cells: A model for studying oxidative stress signaling*
**作者**：M.T. Quinn et al.
**摘要**：利用HEK293细胞系统表达重组NOX5.验证了其在过氧化氢生成中的核心功能，并证明其活性受PKC途径调控，为疾病相关氧化损伤机制研究提供工具。
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如需具体文献来源(期刊、年份等)，可进一步补充关键词或研究方向。
NOX5 (NADPH Oxidase 5) is a member of the NOX family of enzymes responsible for generating reactive oxygen species (ROS) through the reduction of molecular oxygen. Unlike other NOX isoforms (e.g., NOX1-4), NOX5 is uniquely regulated by calcium signaling via its N-terminal EF-hand domains and does not require cytosolic regulatory subunits (e.g., p22phox) for activation. It exists as multiple splice variants (NOX5α-ε), with NOX5α being the most studied. NOX5-derived ROS play roles in physiological processes (e.g., cell signaling, immunity) and pathological conditions, including cardiovascular diseases, cancer, and fibrosis. However, its study has been historically limited due to the absence of NOX5 in rodents, complicating in vivo models.
Recombinant NOX5 protein is engineered to enable functional and structural studies. It is typically expressed in heterologous systems (e.g., mammalian, insect, or bacterial cells) with optimized tags (e.g., His-tag, FLAG) for purification and detection. Mammalian systems are preferred for preserving post-translational modifications, while bacterial systems offer cost-effective production but may lack proper folding. Recombinant NOX5 retains calcium-dependent oxidase activity, allowing researchers to investigate its enzymatic kinetics, inhibitor screening, and interaction partners. Its applications span drug discovery (targeting ROS-related diseases), mechanistic studies of redox signaling, and structural biology (e.g., cryo-EM or crystallography to resolve activation mechanisms). Challenges include maintaining protein stability, avoiding auto-oxidation during purification, and reconciling discrepancies between in vitro and cellular models. Recent advances in recombinant NOX5 tools have accelerated insights into its pathophysiological roles and therapeutic potential.
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