| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOX3 |
| Uniprot No | Q9HBY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MMGCWILNEGLSTILVLSWLGINFYLFIDTFYWYEEEESFHYTRVILGSTLAWARASALCLNFNCMLILIPVSRNLISFIRGTSICCRGPWRRQLDKNLRFHKLVAYGIAVNATIHIVAHFFNLERYHWSQSEEAQGLLAALSKLGNTPNESYLNPVRTFPTNTTTELLRTIAGVTGLVISLALVLIMTSSTEFIRQASYELFWYTHHVFIVFFLSLAIHGTGRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTDVFHYPVCVCVAAGIGVTPFAALLKSIWYKCSEAQTPLKLSKVYFYWICRDARAFEWFADLLLSLETRMSEQGKTHFLSYHIFLTGWDENQALHIALHWDENTDVITGLKQKTFYGRPNWNNEFKQIAYNHPSSSIGVFFCGPKALSRTLQKMCHLYSSADPRGVHFYYNKESF |
| 预测分子量 | 64,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOX3重组蛋白的3篇代表性文献示例(注：部分内容为模拟概括，实际文献需通过数据库核实)：
---
1. **文献名称**: "Characterization of Recombinant NOX3 NADPH Oxidase Complex in the Inner Ear"
**作者**: Ueyama T, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了NOX3蛋白，并发现其与p22phox亚基结合后可显著增强ROS生成活性。实验揭示了NOX3在耳蜗组织中的特异性激活机制，为内耳氧化应激相关听力损伤提供了分子基础。
---
2. **文献名称**: "NOX3-Derived Reactive Oxygen Species Induce Cochlear Hair Cell Death"
**作者**: Cheng G, et al.
**摘要**: 通过体外重组NOX3蛋白模型，证实其在噪声暴露下通过ROS介导耳蜗毛细胞凋亡。研究利用siRNA敲低NOX3表达后，显著减少了毛细胞损伤，提示NOX3是治疗噪声性耳聋的潜在靶点。
---
3. **文献名称**: "Structural Insights into NOX3 Activation by Small GTPase Rac1"
**作者**: Kawahara T, et al.
**摘要**: 利用重组NOX3/p22phox复合体的晶体结构解析，揭示了Rac1蛋白通过结合NOX3的特定结构域激活ROS生成的分子机制，为设计NOX3特异性抑制剂提供了结构依据。
---
4. **文献名称**: "NOX3 Recombinant Protein Exacerbates Renal Oxidative Stress in Hypertension Models"
**作者**: Lassegue B, et al.
**摘要**: 研究通过体外表达NOX3重组蛋白，发现其在肾脏近端小管细胞中异常激活ROS通路，导致线粒体功能障碍。动物实验进一步验证NOX3缺失可缓解高血压相关的肾损伤。
---
**备注**：上述文献标题及内容为领域内典型研究方向示例，实际引用时建议通过PubMed或Web of Science以“NOX3 recombinant”等关键词检索近年高质量论文，重点关注其重组表达系统(如哺乳动物/原核细胞)、功能验证及病理机制研究。
**Background of NOX3 Recombinant Protein**
NADPH oxidase 3 (NOX3) is a member of the NADPH oxidase (NOX) family, a group of transmembrane enzymes primarily involved in reactive oxygen species (ROS) generation. Unlike other NOX isoforms, NOX3 is predominantly expressed in the inner ear, particularly in the vestibular system, and plays a critical role in auditory and balance functions. It was first identified due to its association with otoconia formation, essential for gravity sensing in the inner ear. Structurally, NOX3 shares conserved features with other NOX proteins, including six transmembrane domains and cytosolic regions responsible for electron transfer. However, its activity relies heavily on interactions with regulatory subunits (e.g., p22phox, NOXO1. and NOXA1) for proper activation and ROS production.
Recombinant NOX3 protein refers to the engineered form of NOX3 produced in vitro, often using mammalian or insect cell expression systems to ensure proper post-translational modifications. This protein is pivotal for studying NOX3-specific mechanisms, including its role in oxidative stress-related pathologies. Dysregulation of NOX3 has been linked to hearing loss, age-related vestibular dysfunction, and certain cancers. Its recombinant form enables researchers to dissect its enzymatic activity, substrate specificity, and interactions with inhibitors or therapeutic agents.
Research on NOX3 recombinant protein also contributes to understanding broader NOX family biology. Unlike NOX2 (critical in immune defense) or NOX4 (involved in fibrosis), NOX3’s restricted tissue expression makes it a unique target for selective therapeutic interventions. Current studies focus on developing NOX3-specific inhibitors to mitigate oxidative damage in the inner ear or other NOX3-associated diseases while minimizing off-target effects on other NOX isoforms. Overall, NOX3 recombinant protein serves as a vital tool for advancing both basic and translational research in redox biology and related disorders.
×
