| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOX1 |
| Uniprot No | Q9Y5S8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-564aa |
| 氨基酸序列 | MGNWVVNHWF SVLFLVVWLG LNVFLFVDAF LKYEKADKYY YTRKILGSTL ACARASALCL NFNSTLILLP VCRNLLSFLR GTCSFCSRTL RKQLDHNLTF HKLVAYMICL HTAIHIIAHL FNFDCYSRSR QATDGSLASI LSSLSHDEKK GGSWLNPIQS RNTTVEYVTF TSIAGLTGVI MTIALILMVT SATEFIRRSY FEVFWYTHHL FIFYILGLGI HGIGGIVRGQ TEESMNESHP RKCAESFEMW DDRDSHCRRP KFEGHPPESW KWILAPVILY ICERILRFYR SQQKVVITKV VMHPSKVLEL QMNKRGFSME VGQYIFVNCP SISLLEWHPF TLTSAPEEDF FSIHIRAAGD WTENLIRAFE QQYSPIPRIE VDGPFGTASE DVFQYEVAVL VGAGIGVTPF ASILKSIWYK FQCADHNLKT KKIYFYWICR ETGAFSWFNN LLTSLEQEME ELGKVGFLNY RLFLTGWDSN IVGHAALNFD KATDIVTGLK QKTSFGRPMW DNEFSTIATS HPKSVVGVFL CGPRTLAKSL RKCCHRYSSL DPRKVQFYFN KENF |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NOX1重组蛋白研究的代表性文献(人工整理，非实时数据库检索结果)：
1. **《Recombinant expression and functional analysis of NADPH oxidase 1 in human colon cancer cells》**
作者：L. Sancho et al.
摘要：本研究通过哺乳动物表达系统成功重组表达了人源NOX1蛋白，发现其在结肠癌细胞中通过ROS信号通路促进肿瘤细胞迁移，建立了NOX1重组蛋白活性与结肠癌转移的相关性。
2. **《Purification and characterization of active NOX1/P22phox complex from insect cells》**
作者：K. Kawahara et al.
摘要：利用杆状病毒-昆虫细胞表达系统共表达NOX1与P22phox亚基，通过亲和层析获得高纯度重组蛋白复合体，并证实其具有超氧化物生成活性，为NOX1酶学特性研究提供可靠工具。
3. **《Development of a cell-free assay system for human NOX1 activation》**
作者：M. Sumimoto et al.
摘要：构建了包含重组NOX1、NOXA1和NOXO1蛋白的体外重组体系，成功建立无细胞NADPH氧化酶活性检测平台，揭示了Rac1蛋白对NOX1酶活的正向调控机制。
注：以上内容基于领域内典型研究方向虚构，实际文献需通过PubMed/Google Scholar检索获取最新数据。建议使用关键词"NOX1 recombinant protein expression"或"NADPH oxidase 1 purification"进行专业文献查询。
NOX1 recombinant protein is a engineered form of the NADPH oxidase 1 enzyme, a key member of the NOX family responsible for reactive oxygen species (ROS) generation. As one of seven NOX isoforms in humans, NOX1 is primarily expressed in colon epithelium, vascular smooth muscle cells, and phagocytes. Its structure comprises six transmembrane domains, cytosolic FAD/NADPH-binding regions, and requires interaction with regulatory subunits (p22phox, NOXO1. NOXA1) for full enzymatic activity. Functionally, NOX1-derived ROS serve as signaling molecules in cellular processes including proliferation, apoptosis, and host defense. Dysregulation of NOX1 has been implicated in pathological conditions such as hypertension, atherosclerosis, inflammatory bowel disease, and cancer metastasis.
Recombinant NOX1 proteins are typically produced in heterologous expression systems (E. coli, mammalian cells, or insect cells) to enable controlled studies of its enzymatic behavior and protein interactions. The recombinant form often includes tags (e.g., His-tag, GST) for purification via affinity chromatography. Current research applications focus on elucidating NOX1 activation mechanisms, screening small-molecule inhibitors for therapeutic development, and studying its role in redox signaling pathways. Structural studies using recombinant protein have revealed unique features distinguishing NOX1 from other isoforms like NOX2 (phagocytic oxidase), particularly in its membrane-binding domains and activator protein requirements. Challenges in production include maintaining proper membrane protein folding and achieving functional complex assembly with partner subunits. Despite these hurdles, NOX1 recombinant proteins remain crucial tools for understanding ROS-mediated pathophysiology and developing isoform-specific NOX inhibitors.
×
