| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPPED1 |
| Uniprot No | Q9BRF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSAAEAGGVFHRARGRTLAAFPAEKES EWKGPFYFILGADPQFGLIKAWSTGDCDNGGDEWEQEIRLTEQAVQAINK LNPKPKFFVLCGDLIHAMPGKPWRTEQTEDLKRVLRAVDRAIPLVLVSGN HDIGNTPTAETVEEFCRTWGDDYFSFWVGGVLFLVLNSQFYENPSKCPSL KQAQDQWLDEQLSIARQRHCQHAIVFQHIPLFLESIDEDDDYYFNLSKST RKKLADKFIHAGVKVVFSGHYHRNAGGTYQNLDMVVSSAIGCQLGRDPHG LRVVVVTAEKIVHRYYSLDELSEKGIEDDLMDLIKKK |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CPPED1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*CPPED1 suppresses the inflammatory response of breast cancer cells by inhibiting the calcineurin-NFAT pathway*
**作者**：Li, Y., et al.
**摘要**：该研究通过重组表达人源CPPED1蛋白，发现其能够通过抑制钙调磷酸酶(calcineurin)活性，阻断NFAT信号通路的激活，从而降低乳腺癌细胞中炎症因子IL-6和TNF-α的分泌，为靶向治疗提供潜在靶点。
2. **文献名称**：*Structural and functional characterization of recombinant CPPED1 as a novel regulator of Akt signaling*
**作者**：Wang, X., et al.
**摘要**：作者利用大肠杆菌系统成功表达并纯化重组CPPED1蛋白，通过晶体结构解析发现其具有独特的磷酸酶样结构域，并证实其通过去磷酸化Akt(蛋白激酶B)抑制肿瘤细胞的增殖和迁移。
3. **文献名称**：*CPPED1 deficiency promotes neuronal survival via mTORC1 signaling modulation: Insights from recombinant protein rescue experiments*
**作者**：Zhang, H., et al.
**摘要**：研究通过体外重组CPPED1蛋白回补实验，证明其在神经元中通过调控mTORC1通路活性减少氧化应激诱导的细胞凋亡，提示CPPED1可能作为神经退行性疾病的治疗分子。
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**备注**：以上文献为虚拟示例，实际研究中建议通过PubMed或Web of Science以关键词“CPPED1 recombinant protein”或“CPPED1 function”检索真实文献。
**Background of CPPED1 Recombinant Protein**
CPPED1 (Calcinerin-like phosphoesterase domain-containing protein 1), also known as CPD1. is a metallo-dependent protein phosphatase belonging to the calcineurin-like phosphoesterase (CPPED) family. It is characterized by a conserved catalytic domain that shares structural homology with calcineurin, a calcium/calmodulin-regulated phosphatase involved in immune and neuronal signaling. Unlike calcineurin, CPPED1 lacks regulatory subunits and exhibits distinct substrate specificity, suggesting unique functional roles in cellular processes.
The protein is encoded by the *CPPED1* gene, located on human chromosome 2. and is expressed in various tissues, including the brain, testis, and immune cells. Studies indicate that CPPED1 may regulate cell cycle progression, apoptosis, and stress responses by modulating phosphorylation-dependent signaling pathways. Notably, it has been implicated in dephosphorylating specific residues on key regulatory proteins, such as Akt (protein kinase B), influencing cell survival and metabolism. Dysregulation of CPPED1 has been linked to pathological conditions, including cancer, neurodegenerative disorders, and metabolic syndromes, highlighting its potential as a therapeutic target.
Recombinant CPPED1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional enzyme for research. This tool enables mechanistic studies of its phosphatase activity, substrate interactions, and structural properties. Applications range from elucidating its role in cellular signaling to screening small-molecule modulators for drug development. By leveraging recombinant CPPED1. researchers aim to unravel its physiological significance and explore its diagnostic or therapeutic potential in disease contexts.
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