| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNJ10 |
| Uniprot No | P78508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 276-379aa |
| 氨基酸序列 | DFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIA DFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVR ISNV |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCNJ10重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**: "Inwardly Rectifying Potassium Channels: Their Structure, Function, and Physiological Roles"
**作者**: Hibino, H., et al.
**摘要**: 本研究通过重组表达KCNJ10蛋白，分析了其在调节细胞膜电位和钾离子稳态中的作用，揭示了其与神经系统及肾脏功能相关的分子机制。
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2. **文献名称**: "Characterization of Recombinant KCNJ10/Kir4.1 Channels Stably Expressed in HEK293 Cells"
**作者**: D'Adamo, M.C., et al.
**摘要**: 通过在HEK293细胞中稳定表达重组KCNJ10蛋白，研究其电生理特性，发现其与神经元兴奋性和胶质细胞钾离子缓冲功能密切相关。
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3. **文献名称**: "Functional Analysis of a Missense Mutation in KCNJ10 Linked to Seizures and Developmental Delay"
**作者**: Freudenthal, B., et al.
**摘要**: 通过重组KCNJ10蛋白的功能研究，揭示了特定突变导致钾通道功能异常，进而引发癫痫和神经发育障碍的病理机制。
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如需更具体的文献，建议通过PubMed或Google Scholar以“KCNJ10 recombinant protein”为关键词检索最新研究。
KCNJ10. also known as Kir4.1. is an inwardly rectifying potassium channel encoded by the KCNJ10 gene. It plays a critical role in maintaining potassium ion homeostasis and regulating membrane potential in various tissues. Structurally, it belongs to the Kir channel family, characterized by two transmembrane domains flanking a pore-forming region. KCNJ10 forms functional homotetramers or heterotetramers with related subunits like Kir5.1 (KCNJ16), enabling tissue-specific regulation of potassium flux. It is predominantly expressed in the brain (astrocytes, oligodendrocytes), kidneys (distal nephron), inner ear, and retina, where it contributes to extracellular K⁺ buffering, neuronal excitability, and electrolyte transport.
Dysfunction of KCNJ10 is linked to human diseases. Loss-of-function mutations cause EAST/SeSAME syndrome, a rare autosomal recessive disorder featuring epilepsy, ataxia, sensorineural deafness, and renal tubulopathy. Its role in neuroprotection and potassium spatial buffering also implicates it in neurodegenerative conditions like Parkinson’s disease and multiple sclerosis.
Recombinant KCNJ10 protein is engineered using heterologous expression systems (e.g., mammalian cells, Escherichia coli) for functional and structural studies. Purified recombinant proteins enable investigations into channel gating mechanisms, pharmacology, and disease-associated mutations. Researchers utilize it to screen modulators targeting potassium channels for therapeutic development. Additionally, it serves as an antigen for antibody production in autoimmune disease research.
The production of recombinant KCNJ10 addresses challenges in studying membrane proteins, offering a controlled platform to dissect its biophysical properties and interactions with regulatory proteins like dystrophin-associated complexes in astrocytes. This tool advances our understanding of potassium channelopathies and supports drug discovery for neurological and renal disorders.
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