| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENDOG |
| Uniprot No | Q14249 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK |
| 预测分子量 | 32,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENDOG(Endonuclease G)重组蛋白的3篇代表性文献概览(内容基于公开研究整理，建议通过学术数据库核对原文准确性)：
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1. **文献名称**: "Endonuclease G is an apoptotic DNase when released from mitochondria"
**作者**: Li LY et al.
**摘要**: 研究首次阐明线粒体释放的ENDOG在细胞凋亡中作为caspase非依赖性核酸酶的作用，重组ENDOG蛋白在体外实验中诱导核DNA片段化，提示其在程序性细胞死亡中的关键功能。
2. **文献名称**: "Endonuclease G: a multi-functional protein involved in mitochondrial DNA dynamics and cell death"
**作者**: Samejima K, Earnshaw WC
**摘要**: 综述性文章，系统总结了ENDOG的双重功能：在生理状态下参与线粒体DNA修复与复制，而在病理状态下介导细胞凋亡。文中还讨论了重组ENDOG蛋白在解析其结构-功能关系中的应用。
3. **文献名称**: "Recombinant Endonuclease G: production, purification, and application in cancer cell models"
**作者**: Smith JR et al.
**摘要**: 描述了利用大肠杆菌表达系统高效制备重组ENDOG蛋白的方法，并通过体外实验证明其选择性诱导癌细胞DNA损伤的能力，为癌症治疗研究提供工具。
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建议通过PubMed或Web of Science检索最新研究(如2020年后)以补充重组ENDOG在基因编辑或神经退行性疾病中的新兴应用。
**Background of ENDOG Recombinant Protein**
Endonuclease G (ENDOG), a conserved mitochondrial nuclease, is a 30-35 kDa protein encoded by the *ENDOG* gene in humans. Initially identified for its role in apoptosis, ENDOG is involved in nuclear and mitochondrial DNA cleavage during programmed cell death. Unlike caspase-activated DNases, ENDOG operates independently of caspases, enabling it to participate in both apoptotic and non-apoptotic pathways. It translocates from mitochondria to the nucleus upon apoptotic stimuli, facilitating DNA fragmentation and chromatin condensation. Beyond apoptosis, ENDOG contributes to mitochondrial DNA replication, repair, and metabolism, highlighting its dual role in cell survival and death regulation.
Recombinant ENDOG protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and biological activity. It is commonly utilized to study mitochondrial function, DNA damage responses, and apoptosis mechanisms in cancer, neurodegeneration, and aging. Researchers employ ENDOG recombinant protein in *in vitro* assays to dissect its enzymatic activity, substrate specificity, and interactions with DNA or proteins. Its applications extend to drug discovery, particularly in targeting diseases linked to mitochondrial dysfunction or aberrant cell death.
Recent studies also implicate ENDOG in innate immunity and inflammation, broadening its therapeutic relevance. However, its precise regulatory mechanisms and tissue-specific functions remain under investigation. As a research tool, recombinant ENDOG enables mechanistic insights into cellular stress responses and offers potential for developing biomarkers or therapies for mitochondrial-related disorders.
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