| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENG |
| Uniprot No | P17813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-658aa |
| 氨基酸序列 | MDRGTLPLAVALLLASCSLSPTSLAETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSKREPVVAVAAPASSESSSTNHSIGSTQSTPCSTSSMA |
| 预测分子量 | 70,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENG(Endoglin)重组蛋白的模拟参考文献示例(仅供参考格式，实际文献需通过学术数据库检索获取)：
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1. **标题**: *Expression and Functional Characterization of Recombinant Human Endoglin in Mammalian Cells*
**作者**: Smith A, et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达了重组人Endoglin蛋白，并通过亲和层析纯化。功能实验表明，该重组蛋白可特异性结合TGF-β1.并抑制内皮细胞的迁移，提示其在调控血管生成中的潜在应用。
2. **标题**: *Therapeutic Potential of Soluble Recombinant Endoglin in Tumor Angiogenesis*
**作者**: Lee J, et al.
**摘要**: 通过在小鼠肿瘤模型中注射可溶性重组Endoglin蛋白，研究发现其显著抑制肿瘤血管生成并减缓肿瘤生长。机制分析表明，该蛋白通过阻断TGF-β/Smad信号通路发挥作用。
3. **标题**: *Structural Insights into Endoglin-Ligand Interactions via Recombinant Protein Crystallography*
**作者**: García-Ruiz C, et al.
**摘要**: 本研究解析了重组Endoglin胞外域的晶体结构，揭示了其与BMP9和TGF-β1结合的分子机制，为设计靶向Endoglin的疾病治疗策略提供了结构基础。
4. **标题**: *Development of a High-Yield ENG Recombinant Protein Production System in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 报道了一种优化的大肠杆菌表达系统，用于高效生产可溶性重组Endoglin蛋白，并通过功能验证证明其生物活性与天然蛋白相似，为大规模制备提供了可行方案。
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**注意**：以上为模拟文献，实际引用时请通过PubMed、Web of Science等平台检索真实文献，并确保内容与您的研究方向匹配。
**Background of ENG Recombinant Protein**
Endoglin (ENG), a transmembrane glycoprotein, serves as a co-receptor in the transforming growth factor-beta (TGF-β) signaling pathway, predominantly expressed on vascular endothelial cells and mesenchymal stromal cells. It plays a critical role in modulating angiogenesis, vascular remodeling, and maintaining vascular integrity by regulating TGF-β1 and TGF-β3 signaling. ENG binds to ligands such as BMP-9 and BMP-10. influencing cellular responses like proliferation, migration, and extracellular matrix synthesis.
Mutations in the *ENG* gene are linked to hereditary hemorrhagic telangiectasia (HHT), a vascular disorder characterized by arteriovenous malformations. This association underscores ENG's importance in vascular development and disease. Recombinant ENG protein, produced via expression systems like mammalian cells (e.g., CHO cells) or *E. coli*, retains functional domains for research and therapeutic applications. It is engineered with tags (e.g., His-tag) to facilitate purification and detection.
ENG recombinant protein is widely used to study TGF-β/BMP signaling mechanisms, screen anti-angiogenic drugs, and develop diagnostic tools for HHT. In therapeutics, it serves as a decoy receptor to inhibit pathological angiogenesis in cancers or macular degeneration. Additionally, ENG levels in bodily fluids are explored as biomarkers for cardiovascular diseases and fibrotic conditions.
Current research focuses on optimizing ENG recombinant variants for enhanced stability and targeting capabilities, as well as elucidating its role in tumor microenvironments and fibrotic pathways. These efforts aim to advance precision medicine strategies for vascular and inflammatory diseases.
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