| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOR1B |
| Uniprot No | O14657 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-336aa |
| 氨基酸序列 | FEPITV GLAIGAASAI TGYLSYNDIY CRFAECCREE RPLNASALKL DLEEKLFGQH LATEVIFKAL TGFRNNKNPK KPLTLSLHGW AGTGKNFVSQ IVAENLHPKG LKSNFVHLFV STLHFPHEQK IKLYQDQLQK WIRGNVSACA NSVFIFDEMD KLHPGIIDAI KPFLDYYEQV DGVSYRKAIF IFLSNAGGDL ITKTALDFWR AGRKREDIQL KDLEPVLSVG VFNNKHSGLW HSGLIDKNLI DYFIPFLPLE YRHVKMCVRA EMRARGSAID EDIVTRVAEE MTFFPRDEKI YSDKGCKTVQ SRLDFH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOR1B重组蛋白的3-4篇参考文献的简要概括(注：以下文献信息为示例性内容，实际文献可能有所差异)：
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1. **文献名称**: *Expression and Purification of Recombinant TOR1B in E. coli*
**作者**: Smith A, et al.
**摘要**: 该研究报道了通过大肠杆菌表达系统成功重组表达人源TOR1B蛋白，并优化了纯化条件。研究验证了重组蛋白的ATP酶活性，为后续功能研究提供了基础材料。
2. **文献名称**: *Structural Insights into TOR1B and Its Role in ER-associated Degradation*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了TOR1B重组蛋白的三维结构，揭示了其与内质网伴侣蛋白的相互作用界面，并探讨了其在错误折叠蛋白降解通路中的潜在作用。
3. **文献名称**: *TOR1B Dysfunction Disrupts Nuclear Envelope Dynamics in Cellular Models*
**作者**: Gupta R, et al.
**摘要**: 利用重组TOR1B蛋白进行体外功能实验，发现其缺陷会导致核膜形态异常，提示TOR1B可能在维持核膜完整性中起关键作用。
4. **文献名称**: *Functional Characterization of TOR1B Mutants Linked to Neurological Disorders*
**作者**: Park J, et al.
**摘要**: 研究通过重组表达携带疾病相关突变的TOR1B蛋白，发现特定突变会显著降低其ATP水解活性，为理解TOR1B相关神经退行性疾病的分子机制提供了依据。
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如需具体文献，建议通过PubMed或Google Scholar以“TOR1B recombinant protein”“Torsin-1B expression”等关键词检索最新研究。
**Background of TOR1B Recombinant Protein**
TOR1B (Torsin-1B) is a member of the Torsin family of ATPases, classified within the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily. These proteins are characterized by their conserved ATP-binding domain and involvement in diverse cellular processes, including membrane remodeling, protein quality control, and nucleocytoplasmic transport. TOR1B shares structural and functional homology with TOR1A (Torsin-1A), a well-studied protein linked to early-onset dystonia (DYT1) when mutated. Unlike TOR1A, TOR1B is ubiquitously expressed across tissues and is considered a constitutively active ATPase, suggesting a broader role in maintaining cellular homeostasis.
Recombinant TOR1B protein is engineered for in vitro studies to dissect its biochemical properties and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant form retains ATPase activity and enables structural analyses, such as crystallography or cryo-EM, to resolve its conformational dynamics. Research highlights TOR1B’s interaction with cofactors like LAP1 (lamina-associated polypeptide 1) and its potential role in regulating endoplasmic reticulum (ER) and nuclear envelope functions. Studies also explore its compensatory mechanisms in TOR1A-deficient models, offering insights into neurodevelopmental disorders.
Despite progress, TOR1B’s precise physiological role remains unclear. Its recombinant form serves as a critical tool for elucidating Torsin family mechanisms, disease pathways, and therapeutic targets, particularly in disorders involving ER stress or nuclear envelope defects.
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