| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAT1 |
| Uniprot No | Q9H2H9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-487aa |
| 氨基酸序列 | MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDEYIPGTTSLGMSVFNLSNAIMGSGILGLAFALANTGILLFLVLLTSVTLLSIYSINLLLICSKETGCMVYEKLGEQVFGTTGKFVIFGATSLQNTGAMLSYLFIVKNELPSAIKFLMGKEETFSAWYVDGRVLVVIVTFGIILPLCLLKNLGYLGYTSGFSLSCMVFFLIVVIYKKFQIPCIVPELNSTISANSTNADTCTPKYVTFNSKTVYALPTIAFAFVCHPSVLPIYSELKDRSQKKMQMVSNISFFAMFVMYFLTAIFGYLTFYDNVQSDLLHKYQSKDDILILTVRLAVIVAVILTVPVLFFTVRSSLFELAKKTKFNLCRHTVVTCILLVVINLLVIFIPSMKDIFGVVGVTSANMLIFILPSSLYLKITDQDGDKGTQRIWAALFLGLGVLFSLVSIPLVIYDWACSSSSDEGH |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNAT1重组蛋白的3篇参考文献示例(注：文献为模拟示例，非真实存在)：
1. **文献名称**：*Functional Expression and Characterization of Recombinant SNAT1 in HEK293 Cells*
**作者**：Lee et al.
**摘要**：研究在HEK293细胞中成功表达SNAT1重组蛋白，分析其在中性氨基酸转运中的动力学特性，证实其对谷氨酰胺的高亲和力及钠离子依赖性。
2. **文献名称**：*Structural Insights into SNAT1 Through Cryo-EM Analysis of the Recombinant Protein*
**作者**：Zhang et al.
**摘要**：利用冷冻电镜解析SNAT1重组蛋白的三维结构，揭示其底物结合口袋和钠离子协同转运的分子机制，为靶向药物设计提供依据。
3. **文献名称**：*SNAT1 Recombinant Protein Purification and Its Role in Neuronal Amino Acid Homeostasis*
**作者**：Garcia-Rodriguez et al.
**摘要**：通过大肠杆菌系统表达并纯化SNAT1重组蛋白，验证其在神经元细胞中的功能，发现其缺失导致细胞内谷氨酰胺水平异常及代谢紊乱。
4. **文献名称**：*pH-Dependent Transport Activity of Recombinant SNAT1 in Liposome Models*
**作者**：Tanaka et al.
**摘要**：将SNAT1重组蛋白重构至脂质体中，证明其转运活性受pH调控，并发现跨膜结构域关键残基对质子敏感性的影响。
(注：以上文献为虚构示例，实际研究中建议通过PubMed或Google Scholar以关键词“SNAT1 recombinant protein”检索最新成果。)
**Background of SNAT1 Recombinant Protein**
SNAT1 (Sodium-Coupled Neutral Amino Acid Transporter 1), also known as SLC38A1 or SAT1. is a member of the solute carrier (SLC) transporter family. It plays a critical role in cellular amino acid homeostasis by mediating the sodium-dependent uptake of small neutral amino acids, such as alanine, glycine, and proline. SNAT1 is particularly significant in the nervous system, where it facilitates the transport of glutamine—a precursor for neurotransmitters like glutamate and GABA—across neuronal and glial membranes. This transporter is integral to the glutamate-glutamine cycle, supporting synaptic transmission and metabolic crosstalk between neurons and astrocytes.
Dysregulation of SNAT1 has been implicated in neurological disorders, cancer progression, and metabolic diseases. For instance, altered SNAT1 expression may affect tumor cell proliferation due to its role in nutrient sensing and mTOR signaling. In neurodegenerative conditions, impaired glutamine transport could disrupt neurotransmitter synthesis and neuronal survival.
Recombinant SNAT1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, function, and interactions. The purified protein retains native conformational and transport properties, enabling *in vitro* assays to characterize substrate specificity, kinetic parameters, and inhibitor profiles. Tagged variants (e.g., His-tagged) simplify purification and detection. Research applications include drug discovery (e.g., targeting amino acid metabolism in cancer), mechanistic studies of transport regulation, and disease modeling.
Overall, SNAT1 recombinant protein serves as a vital tool for deciphering amino acid transporter biology and developing therapeutic strategies for related pathologies.
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