| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCCT |
| Uniprot No | P55017 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1021aa |
| 氨基酸序列 | MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ |
| 预测分子量 | 113 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCCT(钠氯协同转运体，SLC12A3)重组蛋白研究的参考文献示例(仅供参考，实际文献请通过学术数据库检索)：
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1. **"Expression and Purification of Functional Human Sodium-Chloride Cotransporter (hNCCT) in Baculovirus-Infected Insect Cells"**
*作者：Zhang Y, et al.*
**摘要**：报道了利用杆状病毒-昆虫细胞系统高效表达功能性人源NCCT重组蛋白，并通过亲和层析和凝胶过滤纯化，验证其离子转运活性，为药物筛选提供工具。
2. **"Structural Insights into NCCT Regulation by Phosphorylation via Cryo-EM"**
*作者：Chen L, Wang H, et al.*
**摘要**：利用冷冻电镜解析了重组NCCT蛋白的磷酸化修饰结构，揭示了其构象变化对离子转运功能的调控机制，为高血压相关疾病治疗提供新靶点。
3. **"Functional Characterization of Recombinant NCCT in Xenopus Oocytes: Role in Hypertension Pathogenesis"**
*作者：Kim S, et al.*
**摘要**：在非洲爪蟾卵母细胞中表达重组NCCT蛋白，证明特定突变体导致离子转运功能异常，阐明了NCCT失活与遗传性低钾血症的关联。
4. **"Development of a High-Throughput Assay for NCCT Inhibitors Using Recombinant Protein"**
*作者：Smith J, et al.*
**摘要**：基于重组NCCT蛋白建立高通量药物筛选平台，鉴定出多个小分子抑制剂，为利尿剂研发提供先导化合物。
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**说明**：以上为模拟文献，实际研究需查询PubMed、Web of Science等平台，关键词建议：**"SLC12A3 recombinant"**, **"NCCT protein purification"**, **"sodium-chloride cotransporter expression"**。
**Background of NCCT Recombinant Protein**
The NCCT (Na⁺-Cl⁻-dependent Cotransporter), also known as the thiazide-sensitive Na⁺/Cl⁻ cotransporter (NCC), is a membrane protein critical for ion homeostasis in the kidney. It plays a central role in reabsorbing sodium and chloride from the renal tubule, regulating blood pressure, and maintaining electrolyte balance. Dysregulation of NCCT is linked to disorders such as Gitelman syndrome (caused by NCC mutations) and hypertension.
Recombinant NCCT protein refers to the engineered form of this transporter produced via genetic engineering techniques. Its production typically involves cloning the *SLC12A3* gene (encoding NCCT) into expression vectors, followed by transfection into host systems like mammalian cells (e.g., HEK293) or insect cells (e.g., Sf9) to ensure proper post-translational modifications. Purification methods, such as affinity chromatography, yield functional NCCT for research.
Studying recombinant NCCT enables detailed structural and functional analyses, including its phosphorylation-dependent regulation, interaction with thiazide diuretics, and response to hormonal signals (e.g., aldosterone). It also aids in drug discovery for hypertension and genetic kidney diseases. Challenges in its production include preserving membrane protein stability and mimicking native lipid environments. Advances in cryo-EM and mutagenesis have further elucidated its 3D structure and transport mechanisms, enhancing therapeutic targeting. Recombinant NCCT remains a vital tool for understanding renal physiology and developing precision therapies.
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