| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKCC2 |
| Uniprot No | Q13621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1099aa |
| 氨基酸序列 | MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS |
| 预测分子量 | 121 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKCC2重组蛋白的模拟参考文献示例(仅供学术写作参考，非真实文献)：
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1. **标题**：*Functional Characterization of Recombinant NKCC2 Expressed in HEK293 Cells*
**作者**：Smith A, et al.
**摘要**：本研究通过HEK293细胞系统成功表达了重组NKCC2蛋白，并验证其离子转运活性。实验表明，该重组蛋白对呋塞米敏感，且磷酸化修饰可增强其转运效率，为研究NKCC2功能调控提供了模型。
2. **标题**：*Cryo-EM Structure of the NKCC2 Transporter in a Phosphorylated State*
**作者**：Zhang L, et al.
**摘要**：通过冷冻电镜解析了重组NKCC2蛋白的磷酸化状态三维结构，揭示了其离子结合位点及构象变化机制，为开发靶向NKCC2的利尿药物提供了结构基础。
3. **标题**：*Expression and Purification of NKCC2 for Drug Interaction Studies*
**作者**：Tanaka K, et al.
**摘要**：利用杆状病毒-昆虫细胞系统大规模表达并纯化功能性NKCC2重组蛋白，结合放射性同位素示踪技术，筛选了多种抑制剂，确认了特定化合物对NKCC2活性的抑制作用。
4. **标题**：*Role of NKCC2 Mutants in Bartter Syndrome: Insights from Recombinant Protein Assays*
**作者**：Chen R, et al.
**摘要**：通过构建巴特综合征相关的NKCC2突变体重组蛋白，发现特定突变导致离子转运功能丧失，阐明了疾病分子机制，并为基因治疗策略提供了实验依据。
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**注**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索真实文献(关键词：NKCC2/SLC12A1. recombinant protein, ion transport)。
NKCC2 (Na⁺-K⁺-2Cl⁻ cotransporter 2), encoded by the *SLC12A1* gene, is an electroneutral ion transporter primarily expressed in the thick ascending limb of the kidney’s loop of Henle. It plays a critical role in renal electrolyte homeostasis by mediating the reabsorption of sodium, potassium, and chloride ions from urine into epithelial cells, driven by the sodium gradient established by Na⁺/K⁺-ATPase. This process is essential for maintaining body fluid balance, blood pressure regulation, and urine concentration. NKCC2’s activity is tightly regulated by phosphorylation, hormonal signals (e.g., vasopressin), and cellular volume changes.
Mutations in *SLC12A1* are linked to Bartter syndrome type II, a rare autosomal recessive disorder characterized by salt wasting, hypokalemia, and metabolic alkalosis. To study its molecular mechanisms and pathophysiology, recombinant NKCC2 proteins are engineered using heterologous expression systems (e.g., mammalian cells, Xenopus oocytes). These recombinant variants enable functional characterization of transport kinetics, ion specificity, and responses to inhibitors like loop diuretics (e.g., furosemide), which target NKCC2 to treat hypertension and edema.
Recent structural studies using cryo-EM and homology modeling have revealed insights into NKCC2’s transmembrane topology and ion-binding sites, advancing drug design efforts. Recombinant NKCC2 also serves as a tool for screening therapeutic candidates and investigating regulatory pathways, bridging basic research to clinical applications in renal and cardiovascular diseases.
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