| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPEB1 |
| Uniprot No | Q9BZB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLGKPPLSFLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLSLSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS |
| 预测分子量 | 69.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CPEB1重组蛋白的关键参考文献(虚构示例，仅供格式参考)：
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1. **文献名称**: *Crystal structure of the RNA-binding domain of CPEB1 reveals conserved phosphorylation motifs*
**作者**: Hake LE, et al.
**摘要**: 本研究利用大肠杆菌重组表达了CPEB1的RNA结合结构域(氨基酸100-250)，通过X射线晶体学解析其三维结构，揭示了其锌指结构域中保守的丝氨酸磷酸化位点，为解释CPEB1在mRNA翻译调控中的作用机制提供结构基础。
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2. **文献名称**: *Recombinant CPEB1 regulates cytoplasmic polyadenylation in vitro through sequence-specific RNA binding*
**作者**: Piqué M, et al.
**摘要**: 作者在HEK293细胞中表达并纯化重组人源CPEB1蛋白，通过体外凝胶迁移实验(EMSA)证明其特异性结合细胞质多聚腺苷酸化元件(CPE)，并激活报告mRNA的poly(A)尾延长，证实CPEB1直接调控翻译起始。
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3. **文献名称**: *CPEB1 overexpression promotes hepatocellular carcinoma via VEGF mRNA stabilization*
**作者**: Huang Y, et al.
**摘要**: 研究通过昆虫杆状病毒系统制备重组CPEB1蛋白，发现其结合VEGF mRNA的3'UTR并增强稳定性。在肝癌细胞中过表达重组CPEB1显著促进血管生成和肿瘤生长，提示其作为癌症治疗靶点的潜力。
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(注：以上文献为模拟内容，实际引用请通过PubMed/Google Scholar检索关键词如"CPEB1 recombinant protein"或"CPEB1 purification"获取真实数据。)
CPEB1 (Cytoplasmic Polyadenylation Element-Binding Protein 1) is an RNA-binding protein that plays a critical role in post-transcriptional regulation of gene expression, particularly in neurons and germ cells. It binds to cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated regions (UTRs) of target mRNAs, regulating their translation through polyadenylation and deadenylation. This mechanism is essential for synaptic plasticity, long-term memory formation, and early embryonic development. Structurally, CPEB1 contains two RNA recognition motifs (RRMs) and a zinc finger domain, enabling sequence-specific RNA interactions.
Recombinant CPEB1 protein is engineered for in vitro studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), recombinant CPEB1 allows researchers to analyze its RNA-binding kinetics, structural dynamics, and interactions with translational machinery. Studies using recombinant protein have revealed its role in phase separation, forming ribonucleoprotein granules critical for localized mRNA translation. Dysregulation of CPEB1 is linked to neurological disorders (e.g., Alzheimer’s disease) and cancer, where it modulates oncogene and tumor suppressor mRNAs. Recombinant variants (e.g., phosphomimetic mutants) help explore post-translational modifications regulating its activity. This tool has advanced therapeutic discovery, enabling high-throughput screens for small molecules targeting CPEB1 in disease contexts. Overall, recombinant CPEB1 serves as a cornerstone for unraveling its biological significance and therapeutic potential.
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