| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NIS |
| Uniprot No | Q92911 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-643aa |
| 氨基酸序列 | MEAVETGERPTFGAWDYGVFALMLLVSTGIGLWVGLARGGQRSAEDFFTGGRRLAALPVGLSLSASFMSAVQVLGVPSEAYRYGLKFLWMCLGQLLNSVLTALLFMPVFYRLGLTSTYEYLEMRFSRAVRLCGTLQYIVATMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTFYTAVGGMKAVVWTDVFQVVVMLSGFWVVLARGVMLVGGPRQVLTLAQNHSRINLMDFNPDPRSRYTFWTFVVGGTLVWLSMYGVNQAQVQRYVACRTEKQAKLALLINQVGLFLIVSSAACCGIVMFVFYTDCDPLLLGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSGTLSTASTSINAMAAVTVEDLIKPRLRSLAPRKLVIISKGLSLIYGSACLTVAALSSLLGGGVLQGSFTVMGVISGPLLGAFILGMFLPACNTPGVLAGLGAGLALSLWVALGATLYPPSEQTMRVLPSSAARCVALSVNASGLLDPALLPANDSSRAPSSGMDASRPALADSFYAISYLYYGALGTLTTVLCGALISCLTGPTKRSTLAPGLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCVGHDGGRDQQETNL |
| 预测分子量 | 68,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NIS(钠碘同向转运体)重组蛋白的3-4篇参考文献及其摘要概括：
1. **Dai, G., Levy, O., & Carrasco, N. (1996).**
*Cloning and characterization of the thyroid iodide transporter.* **Nature**
摘要：首次成功克隆并鉴定了人类NIS基因，通过哺乳动物细胞中的重组表达证实其介导碘摄取的活性，为后续甲状腺疾病治疗及放射性碘应用奠定分子基础。
2. **Spitzweg, C., et al. (2000).**
*In vivo sodium iodide symporter gene therapy of prostate cancer.* **Gene Therapy**
摘要：利用腺病毒载体在前列腺癌细胞中重组表达NIS蛋白，诱导肿瘤细胞特异性摄取放射性碘，探索NIS作为基因治疗靶点用于非甲状腺癌症的潜力。
3. **De la Vieja, A., et al. (2000).**
*Molecular analysis of the sodium/iodide symporter: impact on thyroid and extrathyroid pathophysiology.* **Physiological Reviews**
摘要：系统综述NIS的结构与功能研究进展，涵盖重组蛋白技术在解析其转运机制、突变关联疾病及靶向治疗中的应用。
4. **Wapnir, I. L., et al. (2003).**
*Functional characterization of the sodium/iodide symporter in breast cancer cells.* **Cancer Research**
摘要：通过在乳腺癌细胞中重组表达NIS蛋白，证实其介导碘摄取的能力，为乳腺癌的放射性碘治疗提供实验依据。
这些文献涵盖了NIS重组蛋白的克隆、功能研究及在基因治疗和癌症治疗中的应用方向。
The sodium/iodide symporter (NIS), a transmembrane glycoprotein encoded by the *SLC5A5* gene, plays a critical role in iodide uptake, primarily in the thyroid gland. By coupling the transport of iodide (I⁻) with sodium ions (Na⁺) across the cell membrane, NIS enables the concentration of iodide necessary for thyroid hormone synthesis. Beyond its physiological function, NIS has garnered significant attention in biomedical research and therapeutic applications, particularly in oncology. Its ability to mediate radioactive iodine (¹³¹I) uptake forms the basis of diagnostic imaging and targeted radiotherapy for thyroid cancers. However, many non-thyroid cancers lack endogenous NIS expression, limiting this approach.
Recombinant NIS proteins, produced via genetic engineering in heterologous systems (e.g., mammalian cells, bacteria, or yeast), are pivotal for studying NIS structure-function relationships, regulatory mechanisms, and drug interactions. These proteins retain the ability to transport iodide when expressed in non-thyroid cells, enabling their use in novel therapeutic strategies. For instance, gene therapy approaches deliver NIS-encoding vectors to cancer cells, rendering them susceptible to ¹³¹I therapy. Recombinant NIS also serves as a tool for optimizing iodide uptake efficiency, exploring post-translational modifications, and screening pharmacological modulators.
Challenges persist, including ensuring stable expression, avoiding immunogenicity, and enhancing targeting specificity. Nevertheless, recombinant NIS technology bridges basic science and clinical innovation, offering potential for personalized cancer treatment and expanding the scope of radioisotope-based therapies beyond thyroid disorders. Its versatility underscores its growing importance in precision medicine and molecular imaging.
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