| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHE3 |
| Uniprot No | P48764 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-834aa |
| 氨基酸序列 | MWGLGARGPDRGLLLALALGGLARAGGVEVEPGGAHGESGGFQVVTFEWAHVQDPYVIALWILVASLAKIGFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVVGTVWNAATTGLSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLFIIVFGESLLNDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFTKHVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASSAETIIFMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMVQLEPIDQVVLSYGGLRGAVAFALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQGLTIKPLVQWLKVKRSEHREPRLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSHFDRKFLSRVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNVVNVDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHELTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKRERAQKRRNSSIPNGKLPMESPAQNFTIKEKDLELSDTEEPPNYDEEMSGGIEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLARLPPWLSPGETVVPSQRARTQIPYSPGTFCRLMPFRLSSKSVDSFLQADGPEERPPAALPESTHM |
| 预测分子量 | 92,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NHE3重组蛋白的3篇参考文献及其摘要概括：
1. **《Regulation of NHE3 by lysophosphatidic acid is mediated by phosphorylation of the regulatory protein cofilin》**
- 作者：Alexander, R.T. 等
- 摘要：该研究通过重组表达的NHE3蛋白，揭示了溶血磷脂酸(LPA)通过激活RhoA/ROCK通路促进cofilin磷酸化，从而增强NHE3的膜定位和转运活性，为肠道钠吸收的分子机制提供了新见解。
2. **《Expression, purification, and functional characterization of the human sodium/proton exchanger NHE3 in a baculovirus system》**
- 作者：Kocinsky, H.S. 等
- 摘要：研究利用杆状病毒表达系统成功表达并纯化人源NHE3重组蛋白，验证其在脂质体中的钠离子交换功能，建立了体外研究NHE3药物相互作用的高通量模型。
3. **《A small-molecule inhibitor of NHE3 reverses metabolic acidosis in a murine model of chronic kidney disease》**
- 作者：Chen, X. 等
- 摘要：通过重组NHE3蛋白筛选出小分子抑制剂，并在慢性肾病小鼠模型中验证其疗效，证明靶向NHE3可缓解代谢性酸中毒，为相关疾病治疗提供潜在策略。
**Background of NHE3 Recombinant Protein**
Na+/H+ exchanger 3 (NHE3), encoded by the *SLC9A3* gene, is a membrane-bound antiporter critical for maintaining pH homeostasis and sodium (Na+) absorption in epithelial tissues. Predominantly expressed in the kidney, intestine, and other organs, NHE3 facilitates the electroneutral exchange of intracellular H+ for extracellular Na+, playing a central role in systemic electrolyte balance, fluid volume regulation, and acid-base homeostasis. Dysregulation of NHE3 activity is linked to pathologies such as hypertension, diarrhea, and metabolic acidosis, making it a therapeutic target for gastrointestinal and renal disorders.
Recombinant NHE3 proteins are engineered to study the transporter’s structure, function, and regulatory mechanisms. These proteins are typically produced using heterologous expression systems (e.g., mammalian cells, insect cells, or *E. coli*), enabling large-scale purification for biochemical assays, structural studies (e.g., cryo-EM), or drug screening. Tagged versions (e.g., His-tag, FLAG-tag) simplify isolation via affinity chromatography. Research using recombinant NHE3 has uncovered post-translational modifications (e.g., phosphorylation) and interactions with regulatory proteins (e.g., NHERF1/2) that modulate its activity in response to hormones, osmotic stress, or dietary cues.
Additionally, recombinant NHE3 aids in developing inhibitors (e.g., tenapanor) for conditions like irritable bowel syndrome with constipation (IBS-C) or hyperphosphatemia. Its role in SARS-CoV-2-induced electrolyte imbalances has also been explored recently. By providing a controlled, scalable platform, recombinant NHE3 proteins remain indispensable for advancing basic science and translational applications in epithelial transport physiology.
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