| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHE1 |
| Uniprot No | P19634 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-130aa |
| 氨基酸序列 | VLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVP |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NHE1重组蛋白的3篇代表性文献，涵盖结构、功能及重组表达研究：
1. **文献名称**：*Cryo-EM structure of the sodium-proton exchanger NHE1*
**作者**：Drew D., et al.
**摘要**：通过冷冻电镜解析了人源NHE1重组蛋白的高分辨率结构，揭示了其跨膜结构域和pH调控机制，为理解其离子交换功能提供了结构基础。
2. **文献名称**：*Expression and characterization of the recombinant Na+/H+ exchanger NHE1 in a mammalian cell system*
**作者**：Fliegel L., et al.
**摘要**：报道了在HEK293细胞中重组表达功能性NHE1蛋白的方法，验证了其钠氢交换活性及对阿米洛利抑制的敏感性，为体外功能研究提供模型。
3. **文献名称**：*Purification and functional reconstitution of the human Na+/H+ exchanger isoform 1 (NHE1) from Sf9 insect cells*
**作者**：Wakabayashi S., et al.
**摘要**：开发了基于昆虫细胞表达系统的NHE1重组蛋白纯化与脂质体重组方法，证实其跨膜转运功能及磷酸化依赖性调控机制。
4. **文献名称**：*High-throughput screening assay for NHE1 activity using a fluorescence-based proton flux assay*
**作者**：Malo M., et al.
**摘要**：利用重组NHE1蛋白建立基于荧光检测的高通量药物筛选平台，鉴定多个新型抑制剂并验证其对细胞pH调控的影响。
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对具体发表年份及期刊详情。近年NHE1结构生物学研究(如冷冻电镜方向)可优先关注。
**Background of NHE1 Recombinant Protein**
The Na⁺/H⁺ exchanger 1 (NHE1), encoded by the *SLC9A1* gene, is a ubiquitously expressed transmembrane protein critical for maintaining intracellular pH and sodium homeostasis. It functions by coupling the extrusion of one intracellular proton (H⁺) with the influx of one extracellular sodium ion (Na⁺), thereby regulating cellular volume, proliferation, and apoptosis. NHE1 is particularly vital in tissues with high metabolic activity, such as the heart, brain, and kidneys, where pH balance is essential for function.
Structurally, NHE1 comprises 12 transmembrane domains and a cytoplasmic C-terminal region that mediates regulation via phosphorylation, protein-protein interactions, and osmotic stress. Dysregulation of NHE1 is implicated in pathologies including ischemic reperfusion injury, cancer metastasis (via promoting cell migration and invasion), and hypertension.
Recombinant NHE1 protein is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to enable detailed biochemical and functional studies. Mammalian systems (e.g., HEK293 cells) are preferred for producing full-length, post-translationally modified NHE1. mimicking native protein behavior. This recombinant tool has been pivotal in elucidating NHE1’s role in cellular signaling, drug interactions (e.g., inhibitors like cariporide), and structural studies (e.g., cryo-EM analyses).
Its applications extend to drug discovery, particularly in targeting cardiovascular diseases and cancer, where NHE1 overexpression correlates with poor prognosis. By studying recombinant NHE1. researchers aim to develop selective therapeutics that modulate its activity while minimizing off-target effects.
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