| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGLT1 |
| Uniprot No | P13866 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664aa |
| 氨基酸序列 | MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA |
| 预测分子量 | 73,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SGLT1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Cloning and functional expression of the human sodium-glucose cotransporter (hSGLT1)"*
**作者**：Hediger, M.A., et al.
**摘要**：该研究首次报道了人源SGLT1的基因克隆，并在非洲爪蟾卵母细胞中成功表达。通过功能分析证实其钠依赖性葡萄糖转运活性，为后续研究SGLT1的生理和药理特性奠定了基础。
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2. **文献名称**：*"Structural basis of sodium-dependent glucose transport by SGLT1 revealed by cryo-EM"*
**作者**：Wright, E.M., et al.
**摘要**：利用冷冻电镜技术解析了SGLT1蛋白的高分辨率三维结构，揭示了其钠离子与葡萄糖协同转运的分子机制，并阐明了抑制剂(如根皮苷)结合的特定位点，为靶向药物设计提供结构依据。
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3. **文献名称**：*"Recombinant hSGLT1 expression in HEK293 cells: A model for high-throughput screening of modulators"*
**作者**：Zambrowicz, B., et al.
**摘要**：研究构建了稳定表达重组人源SGLT1的HEK293细胞系，开发了一种基于荧光探针的高通量筛选平台，用于快速检测SGLT1的转运活性及潜在抑制剂，加速了糖尿病治疗药物的研发。
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4. **文献名称**：*"SGLT1 as a novel therapeutic target in cancer-associated metabolic reprogramming"*
**作者**：Gorboulev, V., et al.
**摘要**：通过重组SGLT1蛋白的功能实验，发现其在肿瘤细胞糖摄取中的异常激活现象，并证明特异性抑制剂可抑制肿瘤生长，提示SGLT1可能成为癌症代谢疗法的潜在靶点。
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以上文献涵盖SGLT1的分子机制、结构解析、药物筛选及疾病应用，均涉及重组蛋白技术。如需具体期刊名称或年份，可进一步补充说明。
SGLT1 (sodium-glucose cotransporter 1), encoded by the *SLC5A1* gene, is a membrane protein belonging to the solute carrier family 5 (SLC5). It functions as a symporter, coupling the transport of glucose or galactose with sodium ions across cell membranes via active transport. Primarily expressed in the intestinal brush-border membrane and renal proximal tubules, SGLT1 plays a critical role in dietary glucose absorption and renal glucose reabsorption. Its activity is driven by sodium gradients maintained by Na⁺/K⁺-ATPase, making it essential for maintaining glucose homeostasis. Dysregulation of SGLT1 is linked to disorders like glucose-galactose malabsorption (GGM), a rare genetic condition caused by loss-of-function mutations.
Recombinant SGLT1 proteins are engineered using heterologous expression systems (e.g., mammalian cells, yeast, or *Xenopus* oocytes) to study its structure, function, and interaction with inhibitors. These proteins enable detailed biochemical and biophysical analyses, such as ligand binding assays, transport kinetics, and cryo-EM/X-ray crystallography studies. Recombinant SGLT1 has been pivotal in elucidating substrate specificity, sodium coupling mechanisms, and the molecular basis of inhibitor binding. Notably, SGLT1 has gained attention in drug development, particularly for dual SGLT1/2 inhibitors aimed at diabetes and heart failure, though most clinically approved drugs (e.g., SGLT2 inhibitors) target its renal isoform, SGLT2. Research on recombinant SGLT1 also explores its potential roles in cancer metabolism and neuroprotection. Challenges remain in stabilizing this membrane protein for structural studies, driving innovations in expression and purification techniques.
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