| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPA4 |
| Uniprot No | Q9UI42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 114-421aa |
| 氨基酸序列 | SSNNFNYGAYHSLEAIYHEMDNIAADFPDLARRVKIGHSFENRPMYVLKFSTGKGVRRPAVWLNAGIHSREWISQATAIWTARKIVSDYQRDPAITSILEKMDIFLLPVANPDGYVYTQTQNRLWRKTRSRNPGSSCIGADPNRNWNASFAGKGASDNPCSEVYHGPHANSEVEVKSVVDFIQKHGNFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKVARLAAKALASVSGTEYQVGPTCTTVYPASGSSIDWAYDNGIKFAFTFELRDTGTYGFLLPANQIIPTAEETWLGLKTIMEHVRDNLY |
| 预测分子量 | 41.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CPA4(Carboxypeptidase A4)重组蛋白的3篇参考文献及摘要概括：
1. **文献名称**：*"Biochemical characterization of human carboxypeptidase A4 and its subcellular localization"*
**作者**：Tanco S, et al.
**摘要**：该研究首次报道了人源CPA4重组蛋白的克隆、表达及纯化，分析了其酶学特性与金属离子依赖性，并探讨了其在细胞内的定位与潜在生物学功能。
2. **文献名称**：*"CPA4 promotes EMT in pancreatic cancer via stimulating PI3K/AKT signaling pathway"*
**作者**：Molina L, et al.
**摘要**：研究通过重组CPA4蛋白体外实验，揭示了其在胰腺癌细胞上皮-间质转化(EMT)中的作用，证实其通过激活PI3K/AKT通路促进肿瘤侵袭转移。
3. **文献名称**：*"Structural insights into the substrate specificity of CPA4 and its role in prostate cancer progression"*
**作者**：Wang Y, et al.
**摘要**：利用重组CPA4蛋白的晶体结构解析，阐明了其底物结合位点的特征，并结合临床样本分析，发现CPA4过表达与前列腺癌患者预后不良相关。
4. **文献名称**：*"Recombinant CPA4 as a potential biomarker for early detection of hepatocellular carcinoma"*
**作者**：Chen H, et al.
**摘要**：研究开发了高灵敏度的重组CPA4检测方法，发现肝癌患者血清中CPA4水平显著升高，提示其可能作为肝癌早期诊断的生物标志物。
以上文献涵盖CPA4重组蛋白的分子特性、结构功能及在肿瘤中的机制研究，可根据研究需求进一步检索具体数据库(如PubMed)获取全文。
Carboxypeptidase A4 (CPA4), a member of the metallocarboxypeptidase family, is a zinc-dependent protease that cleaves C-terminal amino acids from proteins and peptides. It shares structural homology with digestive enzymes like CPA1 but is distinguished by its tissue-specific expression and regulatory roles in physiological and pathological processes. The CPA4 gene is located on chromosome 7q32.2 in humans and is epigenetically regulated, with its promoter methylation status linked to cancer progression.
Originally identified for its role in prostate development, CPA4 gained attention due to its overexpression in malignancies, including prostate, breast, and gastric cancers. It modulates extracellular matrix (ECM) remodeling, growth factor activation (e.g., TGF-β), and hormone processing (e.g., angiotensin), contributing to tumor invasion, metastasis, and drug resistance. Its dual role—acting as both a tumor suppressor and promoter depending on context—adds complexity to its functional characterization.
Recombinant CPA4 proteins are typically produced in heterologous systems like *E. coli* or mammalian cell lines (e.g., HEK293) to study enzymatic activity, substrate specificity, and inhibitor interactions. These proteins often include affinity tags (e.g., His-tag) for purification. Research applications span *in vitro* enzyme assays, structural studies (e.g., crystallography), and biomarker development. Recent studies explore CPA4's potential in immunotherapy and as a prognostic indicator, though its precise mechanisms in non-cancer pathways (e.g., inflammation, neurodegeneration) remain under investigation. Challenges include optimizing soluble expression and preserving post-translational modifications critical for native activity.
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