| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPN |
| Uniprot No | Q9NP59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571aa |
| 氨基酸序列 | MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVV |
| 预测分子量 | 62,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FPN(Ferroportin)重组蛋白研究的代表性文献摘要(注：文献标题与作者为虚拟示例，建议根据实际需求检索真实文献)：
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1. **文献名称**：*Structural basis for iron export by ferroportin*
**作者**：Smith A, et al.
**摘要**：本研究通过X射线晶体学解析了重组人源FPN蛋白的跨膜结构，揭示了其铁离子结合与转运的分子机制，证实了FPN在细胞铁外排中的关键作用及与肝素调控的相互作用。
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2. **文献名称**：*Hepcidin-induced endocytosis of ferroportin is dependent on its ubiquitination*
**作者**：Gao B, et al.
**摘要**：利用重组FPN蛋白体外表达系统，作者证明了铁调节激素hepcidin通过诱导FPN泛素化促进其内吞降解，揭示了铁稳态调控的分子通路，为遗传性血色素沉着症提供了治疗靶点。
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3. **文献名称**：*Recombinant ferroportin expression in HEK293 cells enables functional characterization of disease-associated mutations*
**作者**：Chen L, et al.
**摘要**：通过构建FPN重组表达载体并在HEK293细胞中表达，研究验证了多种遗传突变对FPN铁转运活性的影响，为FPN相关铁代谢疾病的基因诊断提供了实验依据。
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如需具体文献，建议通过PubMed或Google Scholar以关键词 **"ferroportin recombinant protein"** 或 **"FPN structural study"** 检索近年高影响力论文。
Ferroportin (FPN), also known as SLC40A1. is a transmembrane protein critical for systemic iron homeostasis. As the sole known iron exporter in vertebrates, FPN facilitates the release of iron from cells into the bloodstream, particularly from enterocytes (gut iron absorption), hepatocytes (iron storage), and macrophages (recycling aged red blood cells). Its activity is tightly regulated by the peptide hormone hepcidin, which binds to FPN, inducing its internalization and degradation, thereby reducing iron efflux. Dysregulation of FPN function is linked to iron-related disorders, including hereditary hemochromatosis (iron overload) and anemia of chronic disease.
Recombinant FPN proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional forms of the protein for research and therapeutic applications. These proteins enable structural studies (e.g., X-ray crystallography, cryo-EM) to resolve FPN's conformational dynamics and interaction mechanisms with hepcidin or iron ions. They are also used in drug discovery to screen molecules that modulate FPN activity, offering potential treatments for iron metabolism disorders. Additionally, recombinant FPN aids in elucidating genetic mutations affecting iron transport, providing insights into disease mechanisms. Its role in balancing cellular iron export makes it a pivotal target for addressing global health challenges related to iron deficiency and toxicity.
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