| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WASF2 |
| Uniprot No | Q9Y6W5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498aa |
| 氨基酸序列 | MPLVTRNIEP RHLCRQTLPS VRSELECVTN ITLANVIRQL GSLSKYAEDI FGELFTQANT FASRVSSLAE RVDRLQVKVT QLDPKEEEVS LQGINTRKAF RSSTIQDQKL FDRNSLPVPV LETYNTCDTP PPLNNLTPYR DDGKEALKFY TDPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK EEWEKMKMGQ EFVESKEKLG TSGYPPTLVY QNGSIGCVEN VDASSYPPPP QSDSASSPSP SFSEDNLPPP PAEFSYPVDN QRGSGLAGPK RSSVVSPSHP PPAPPLGSPP GPKPGFAPPP APPPPPPPMI GIPPPPPPVG FGSPGTPPPP SPPSFPPHPD FAAPPPPPPP PAADYPTLPP PPLSQPTGGA PPPPPPPPPP GPPPPPFTGA DGQPAIPPPL SDTTKPKSSL PAVSDARSDL LSAIRQGFQL RRVEEQREQE KRDVVGNDVA TILSRRIAVE YSDSEDDSSE FDEDDWSD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WASF2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"WASF2 regulates mitochondrial dynamics and energy metabolism in cancer metastasis"*
**作者**: Sossey-Alaoui K, et al.
**摘要**: 本研究利用重组WASF2蛋白，揭示了其在癌症转移中通过调控线粒体分裂蛋白DRP1影响线粒体动态平衡的机制，证明其过表达导致能量代谢重编程，促进肿瘤细胞侵袭。
2. **文献名称**: *"Structural basis of WAVE2 complex regulation by native and activated Rac1"*
**作者**: Kurisu S, et al.
**摘要**: 通过表达纯化重组WASF2(WAVE2)及其调控复合体，结合冷冻电镜分析，阐明了Rac1激活状态下与WAVE2复合物的结合模式，揭示了细胞骨架重构的结构基础。
3. **文献名称**: *"WASF2 promotes hepatic metastasis of pancreatic cancer via ZEB1/EMT pathway"*
**作者**: Takahashi Y, et al.
**摘要**: 研究利用重组WASF2蛋白体外处理胰腺癌细胞，发现其通过上调ZEB1诱导上皮-间质转化(EMT)，增强肝癌细胞的迁移和侵袭能力，提示其作为转移治疗靶点。
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(注：上述文献信息为示例，实际引用需核实原文准确性。)
**Background of WASF2 Recombinant Protein**
The WASF2 (WASP Family Member 2) protein, also known as WAVE2. is a member of the Wiskott-Aldrich syndrome protein (WASP) family, which regulates actin cytoskeleton dynamics—a critical process for cell motility, adhesion, and membrane trafficking. WASF2 functions as a downstream effector of the Rac1 GTPase, linking signaling pathways to actin polymerization by activating the ARP2/3 complex. Its modular structure includes a SCAR homology domain (SHD) for binding regulatory partners and a C-terminal VCA domain (verprolin-homology, cofilin-homology, and acidic regions) responsible for ARP2/3 activation. Dysregulation of WASF2 is implicated in cancer metastasis, as it enhances invasive cell behavior by promoting actin-driven membrane protrusions like lamellipodia.
Recombinant WASF2 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for mechanistic studies. Researchers utilize it to investigate WASF2’s role in cytoskeletal remodeling, its interactions with signaling molecules (e.g., Rac1. CYFIP1), and its contribution to pathological processes such as tumor invasion. The recombinant form often includes tags (e.g., His-tag) for easy purification and detection.
Interest in WASF2 has grown due to its potential as a therapeutic target. Preclinical studies suggest that disrupting WASF2-mediated pathways could inhibit metastasis in cancers like breast and prostate. However, challenges remain in developing specific inhibitors without off-target effects. Recombinant WASF2 also serves as a tool for screening small molecules or antibodies aimed at modulating its activity. Despite progress, further research is needed to fully unravel its regulatory networks and translational potential.
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