| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX5B |
| Uniprot No | P10606 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-129aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COX5B重组蛋白的模拟参考文献示例，涵盖表达、功能及结构研究：
1. **"高效表达与纯化人源COX5B重组蛋白及其酶活性分析"**
*作者：张某某等*
摘要：研究通过构建pET载体在大肠杆菌中表达COX5B重组蛋白，优化诱导条件并利用亲和层析纯化。经SDS-PAGE和Western blot验证，纯化蛋白具有细胞色素c氧化酶活性，为功能研究奠定基础。
2. **"COX5B重组蛋白调控线粒体呼吸链的分子机制"**
*作者：李某某等*
摘要：通过体外重组COX5B与复合体IV亚基的共孵育实验，发现其可增强复合体IV的稳定性，并利用siRNA敲低模型证实COX5B缺失导致ATP合成减少，提示其在能量代谢中的关键作用。
3. **"COX5B重组蛋白在结直肠癌细胞凋亡中的功能研究"**
*作者：王某某等*
摘要：将重组COX5B导入结直肠癌细胞系后，通过流式细胞术检测到线粒体膜电位下降及凋亡率升高，表明COX5B可能通过调节ROS水平诱导肿瘤细胞死亡。
4. **"基于重组COX5B的X射线晶体结构解析及其底物结合位点鉴定"**
*作者：Smith J等*
摘要：采用昆虫细胞系统表达COX5B重组蛋白，获得2.8Å分辨率晶体结构，揭示其与血红素辅因子的结合模式，突变关键氨基酸导致氧化酶活性丧失，阐明结构-功能关系。
注：以上文献为模拟示例，非真实存在。实际研究中建议通过PubMed或Web of Science以“COX5B recombinant protein”为关键词检索最新文献。
**Background of COX5B Recombinant Protein**
COX5B (Cytochrome c oxidase subunit 5B) is a nuclear-encoded subunit of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (Complex IV). This enzyme catalyzes the transfer of electrons from cytochrome c to molecular oxygen, a critical step in aerobic respiration that contributes to the generation of ATP. COX is a multi-subunit complex, and COX5B, located in the mitochondrial inner membrane, plays a structural and functional role in stabilizing the enzyme’s activity and maintaining its assembly.
The COX5B gene is evolutionarily conserved across eukaryotes, reflecting its essential role in cellular energy metabolism. In humans, COX5B exists as two isoforms (COX5B-1 and COX5B-2) due to alternative splicing, with COX5B-1 being the predominant form in most tissues. Dysregulation of COX5B has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases, highlighting its importance in both physiological and pathological contexts.
Recombinant COX5B protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable studies on its structure, function, and interactions. Purified recombinant COX5B is utilized in biochemical assays to investigate COX assembly mechanisms, enzyme kinetics, or the impact of mutations on oxidative phosphorylation. It also serves as a tool for developing diagnostic antibodies or exploring therapeutic strategies targeting mitochondrial dysfunction.
Research involving recombinant COX5B often addresses questions about tissue-specific isoform expression, hypoxia responses, or metabolic adaptations in disease. However, challenges remain in replicating post-translational modifications (e.g., phosphorylation) inherent to the native protein, which may affect its functional studies. Overall, COX5B recombinant protein remains a vital resource for advancing our understanding of mitochondrial biology and associated diseases.
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