| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IgD |
| Uniprot No | P0DOX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | RLQLQESGPGLVKPSETLSLTCIVSGGPIRRTGYYWGWIRQPPGKGLEWIGGVYYTGSIYYNPSLRGRVTISVDTSRNQFSLNLRSMSAADTAMYYCARGNPPPYYDIGTGSDDGIDVWGQGTTVHVSSAPTKAPDVFPIISGCRHPKDNSPVVLACLITGYHPTSVTVTWYMGTQSQPQRTFPEIQRRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVTDHGPM |
| 预测分子量 | 56,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IgD重组蛋白的3篇代表性文献示例(注：文献为虚构，仅供格式参考)：
1. **文献名称**：*Expression and Functional Analysis of Recombinant IgD in B Cell Activation*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组IgD，证实其可通过结合B细胞表面受体，增强抗原呈递和促炎细胞因子分泌。
2. **文献名称**：*Structural Characterization of Recombinant IgD and Its Role in Immune Regulation*
**作者**：Li X, et al.
**摘要**：通过X射线晶体学解析重组IgD的胞外域结构，发现其铰链区灵活性对B细胞耐受调控至关重要，为自身免疫疾病治疗提供新靶点。
3. **文献名称**：*Recombinant IgD-Fc Fusion Protein as a Potential Therapeutic Agent in Allergic Models*
**作者**：Garcia R, et al.
**摘要**：开发IgD-Fc重组融合蛋白，证明其可通过抑制IgE介导的肥大细胞脱颗粒，缓解小鼠过敏性哮喘症状。
4. **文献名称**：*High-Yield Production of Recombinant IgD in Mammalian Cells and Its Application in Diagnostics*
**作者**：Wang T, et al.
**摘要**：优化CHO细胞表达系统实现重组IgD的高效分泌，并验证其在ELISA检测中用于早期类风湿关节炎标志物筛查的可行性。
(注：以上文献为模拟内容，实际引用请查询真实数据库。)
**Background of IgD Recombinant Protein**
Immunoglobulin D (IgD) is a unique antibody isotype primarily expressed on the surface of mature B lymphocytes as part of the B-cell receptor (BCR) complex. Discovered in 1965. IgD exists alongside IgM as a co-receptor, yet its biological role has historically been less understood compared to other immunoglobulins like IgG or IgE. Structurally, IgD consists of two heavy (δ) chains and two light chains, forming a monomeric structure with a flexible hinge region that enhances antigen-binding versatility.
IgD plays a dual role in immune regulation. On B cells, it participates in antigen recognition, signal transduction, and cell activation, potentially fine-tuning immune responses. Soluble IgD, though rare, has been detected in serum and mucosal secretions, where it may interact with basophils or mast cells to modulate inflammation. Recent studies suggest its involvement in mucosal immunity, autoimmune disorders, and chronic inflammatory conditions, though mechanistic details remain under investigation.
Recombinant IgD proteins are engineered using mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation. These proteins are critical tools for studying IgD-specific interactions, epitope mapping, and B-cell signaling pathways. They also aid in developing diagnostics for IgD-related pathologies (e.g., IgD myeloma) and therapeutic strategies targeting B-cell malignancies or autoimmune diseases.
Research on IgD recombinant proteins is expanding due to advancements in structural biology and immunotherapy, offering new insights into its enigmatic functions and therapeutic potential.
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