| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLUT3 |
| Uniprot No | P11169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKG NAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLL AVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTAL RGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSA ALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMS QEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAG VQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMT VSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPA AMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIFTGFLITFLAFTFFKV PETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAKETTTNV |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLUT3重组蛋白的3篇参考文献示例(文献名称和作者为虚构，供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant Human GLUT3 in Escherichia coli*
**作者**：Smith A et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功克隆并纯化人源GLUT3重组蛋白，优化了表达条件以提高溶解度，并通过Western blot和功能实验验证其葡萄糖转运活性。
2. **文献名称**：*Structural Insights into GLUT3 Conformational Dynamics by Cryo-EM*
**作者**：Chen L et al.
**摘要**：利用冷冻电镜解析了重组GLUT3蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基及构象变化机制，为靶向GLUT3的药物设计提供了结构基础。
3. **文献名称**：*GLUT3 Overexpression in Cancer Cells Enhances Glycolytic Flux and Promotes Tumor Growth*
**作者**：Wang X et al.
**摘要**：通过重组GLUT3在多种癌细胞系中的过表达实验，证实GLUT3通过增强葡萄糖摄取和糖酵解途径显著促进肿瘤细胞增殖，提示其作为癌症治疗靶点的潜力。
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注：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台检索真实文献(关键词：GLUT3. recombinant protein, glucose transporter)。
**Background of GLUT3 Recombinant Protein**
GLUT3 (glucose transporter 3), encoded by the *SLC2A3* gene, is a high-affinity glucose transporter primarily expressed in neurons, spermatozoa, and certain cancer cells. As a member of the solute carrier 2A (SLC2A) family, it facilitates glucose uptake across cell membranes via facilitative diffusion. Unlike other GLUT isoforms, GLUT3 exhibits a strong preference for glucose and operates efficiently under low glucose conditions, making it critical for maintaining energy homeostasis in the brain, where neurons rely heavily on glucose as their primary energy source.
Structurally, GLUT3 is a transmembrane protein with 12 membrane-spanning helices, typical of the GLUT family. Its recombinant form is produced using expression systems like mammalian cells or insect cells to ensure proper post-translational modifications and functional folding. Recombinant GLUT3 is purified via affinity chromatography, often incorporating tags (e.g., His-tag) for detection and isolation.
Research on recombinant GLUT3 has advanced understanding of its role in neurological disorders (e.g., Alzheimer’s disease, epilepsy) and metabolic conditions. In cancer biology, GLUT3 overexpression in tumors correlates with aggressive phenotypes, as cancer cells exploit its high glucose uptake capacity to fuel rapid proliferation. Additionally, studies using recombinant GLUT3 have explored its interaction with insulin signaling, hypoxia responses, and potential therapeutic targeting.
The development of GLUT3 recombinant proteins also supports drug screening and structural studies, enabling the design of inhibitors to modulate glucose metabolism in pathological states. Despite progress, challenges remain in mimicking its native membrane environment *in vitro* and elucidating regulatory mechanisms. Overall, GLUT3 recombinant proteins serve as vital tools for dissecting glucose metabolism in health and disease.
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