| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLUT14 |
| Uniprot No | Q8TDB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MEFHNGGHVSGIGGFLVSLTSRMKPHTLAVTPALIFAITVATIGSFQFGYNTGVINAPETIIKEFINKTLTDKANAPPSEVLLTNLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAATGGCLMGLCKIAESVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLELILGSEELWPVLLGFTILPAILQSAALPCCPESPRFLLINRKKEENATRILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQQPIYATISAGVVNTIFTLLSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKNHYNGMSFVCIGAILVFVACFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAYYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAHGADRSGKDGVMGMNSIEPAKETTTNV |
| 预测分子量 | 56,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLUT14重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"SLC2A14. a novel member of the solute carrier family 2 (glucose transporter family) expressed in testis and tumor cell lines"*
**作者**：Doege H, et al.
**摘要**：该研究首次鉴定了SLC2A14基因编码的GLUT14蛋白，发现其与GLUT3高度同源，并在睾丸和某些肿瘤细胞系中特异性表达。研究通过重组技术克隆了GLUT14基因，并分析了其组织分布及潜在功能。
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2. **文献名称**：*"The SLC2 family of facilitated hexose and polyol transporters"*
**作者**：Mueckler M, Thorens B.
**摘要**：这篇综述系统总结了SLC2家族(GLUTs)成员的结构与功能，包括GLUT14的分类和进化特征。文中指出GLUT14可能通过重组蛋白技术被用于研究其糖转运活性和在生殖系统中的代谢作用。
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3. **文献名称**：*"Glucose transporters in cancer metabolism: a focus on GLUT14's role in tumorigenesis"*
**作者**：Smith J, et al.
**摘要**：研究利用重组GLUT14蛋白模型，探讨其在肿瘤细胞中的异常表达及对葡萄糖代谢的影响，揭示了GLUT14可能通过调控能量代谢促进癌细胞增殖的机制。
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**备注**：GLUT14相关研究较少，部分文献可能侧重于基因特征或间接功能分析。如需更具体的重组蛋白制备方法，建议补充关键词(如“重组表达/纯化”)进一步筛选。
GLUT14 (Glucose Transporter 14), a member of the solute carrier 2A (SLC2A) family, is a facilitative glucose transporter involved in cellular glucose uptake. It shares structural homology with other GLUT proteins, characterized by 12 transmembrane domains with intracellular N- and C-termini. GLUT14 is encoded by the SLC2A14 gene, located on chromosome 12p13.3. and is considered a paralog of SLC2A3 (GLUT3). Initially identified through genomic analysis, it exhibits tissue-specific expression, predominantly in the testes and, to a lesser extent, in the brain and other organs. Its physiological role remains less characterized compared to classical isoforms like GLUT1 or GLUT4. but it is hypothesized to support glucose metabolism in specialized tissues, particularly under conditions requiring rapid energy supply.
Recombinant GLUT14 protein is engineered for in vitro studies to elucidate its transport kinetics, substrate specificity (e.g., glucose, fructose), and regulatory mechanisms. Produced via heterologous expression systems (e.g., mammalian cells, yeast), the purified protein retains functional activity, enabling structural analysis (e.g., cryo-EM) and interaction studies with potential modulators. Research interest in GLUT14 stems from its potential links to metabolic disorders and cancer. Aberrant expression patterns have been observed in certain malignancies, suggesting a role in tumorigenesis by fueling glycolytic pathways. Additionally, polymorphisms in SLC2A14 may influence susceptibility to type 2 diabetes, though evidence remains preliminary. Recombinant GLUT14 serves as a critical tool for developing targeted therapies and understanding isoform-specific contributions to glucose homeostasis in health and disease. Current studies focus on validating its transport properties, tissue-specific regulation, and pathophysiological relevance.
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