| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLCE |
| Uniprot No | O94923 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-617aa |
| 氨基酸序列 | NKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN |
| 预测分子量 | 70.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLCE(D-glucuronyl C5-epimerase)重组蛋白的3篇参考文献的简要概括(格式为非正式列举)：
1. **文献名称**：*"Functional characterization of recombinant human D-glucuronyl C5-epimerase"*
**作者**：Li, J.P., et al.
**摘要内容**：该研究成功在大肠杆菌中表达并纯化了重组人源GLCE蛋白，验证了其催化肝素/硫酸乙酰肝素(HS)链中葡萄糖醛酸向艾杜糖醛酸转化的酶活性，揭示了其在HS生物合成中的关键作用。
2. **文献名称**：*"Structural insights into the substrate specificity of D-glucuronyl C5-epimerase"*
**作者**：Rong, J., et al.
**摘要内容**：通过X射线晶体学解析了重组GLCE蛋白的三维结构，结合分子对接实验，阐明了其底物结合域和催化机制，为设计针对HS相关疾病的抑制剂提供了结构基础。
3. **文献名称**：*"Recombinant GLCE regulates breast cancer cell invasion via modulating heparan sulfate sulfation"*
**作者**：Chen, L., et al.
**摘要内容**：研究发现，重组GLCE蛋白过表达可改变乳腺癌细胞的HS硫酸化模式，抑制肿瘤细胞迁移和侵袭，表明GLCE在癌症转移中的潜在治疗价值。
(注：以上文献为示例性内容，实际文献需通过学术数据库检索获取。)
**Background of GLCE Recombinant Protein**
GLCE (glucuronyl C5-epimerase) is a key enzyme involved in the biosynthesis of heparan sulfate (HS), a complex glycosaminoglycan (GAG) that plays critical roles in cell signaling, cell adhesion, and inflammation. HS chains are synthesized on core proteins to form heparan sulfate proteoglycans (HSPGs), which interact with growth factors, cytokines, and extracellular matrix components to regulate physiological and pathological processes. GLCE catalyzes the conversion of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) in HS chains, a critical epimerization step that enhances structural diversity and functional versatility of HS.
Recombinant GLCE protein is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, enabling large-scale production for research and therapeutic applications. Its recombinant form retains enzymatic activity, allowing studies on HS modification mechanisms, structure-function relationships, and interactions with signaling molecules (e.g., FGF, Wnt). Dysregulation of GLCE has been linked to diseases, including cancer, where altered HS sulfation patterns influence tumor progression, angiogenesis, and metastasis. Recombinant GLCE is thus a valuable tool for investigating HS-related pathways and developing HS-targeted therapies.
Research applications include *in vitro* enzyme activity assays, drug screening for GLCE inhibitors/activators, and structural studies using techniques like X-ray crystallography. Its therapeutic potential lies in modulating HS-dependent processes in inflammatory disorders, fibrosis, and cancer. Overall, recombinant GLCE serves as a vital resource for advancing understanding of GAG biology and translational medicine.
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