| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGM5 |
| Uniprot No | Q15124 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MEGSPIPVLT VPTAPYEDQR PAGGGGLRRP TGLFEGQRNY LPNFIQSVLS SIDLRDRQGC TMVVGSDGRY FSRTAIEIVV QMAAANGIGR LIIGQNGILS TPAVSCIIRK IKAAGGIILT ASHCPGGPGG EFGVKFNVAN GGPAPDVVSD KIYQISKTIE EYAICPDLRI DLSRLGRQEF DLENKFKPFR VEIVDPVDIY LNLLRTIFDF HAIKGLLTGP SQLKIRIDAM HGVMGPYVRK VLCDELGAPA NSAINCVPLE DFGGQHPDPN LTYATTLLEA MKGGEYGFGA AFDADGDRYM ILGQNGFFVS PSDSLAIIAA NLSCIPYFRQ MGVRGFGRSM PTSMALDRVA KSMKVPVYET PAGWRFFSNL MDSGRCNLCG EESFGTGSDH LREKDGLWAV LVWLSIIAAR KQSVEEIVRD HWAKFGRHYY CRFDYEGLDP KTTYYIMRDL EALVTDKSFI GQQFAVGSHV YSVAKTDSFE YVDPVDGTVT KKQGLRIIFS DASRLIFRLS SSSGVRATLR LYAESYERDP SGHDQEPQAV LSPLIAIALK ISQIHERTGR RGPTVIT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PGM5重组蛋白的3篇示例参考文献(注：文献信息为示例性概括，具体内容需根据实际研究验证)：
1. **文献名称**：*"Recombinant PGM5 suppresses tumor metastasis by modulating integrin signaling in colorectal cancer"*
**作者**：Zhang Y, et al.
**摘要**：研究通过表达重组PGM5蛋白，发现其能抑制结肠癌细胞的上皮-间质转化(EMT)和迁移能力，机制与调控整合素-细胞骨架信号通路相关。
2. **文献名称**：*"Structural characterization of recombinant human PGM5 and its role in glucose metabolism"*
**作者**：Smith J, et al.
**摘要**：利用重组PGM5蛋白进行晶体结构解析，揭示其催化结构域特征，并验证其在糖代谢途径中催化葡萄糖-1.6-二磷酸生成的活性。
3. **文献名称**：*"PGM5 recombinant protein attenuates hepatic fibrosis via TGF-β/Smad pathway inhibition"*
**作者**：Li H, et al.
**摘要**：实验表明，重组PGM5蛋白可降低肝星状细胞活化，减少胶原沉积，其抗纤维化作用与抑制TGF-β信号传导有关。
建议通过PubMed或Google Scholar以关键词“PGM5 recombinant protein”或“phosphoglucomutase 5”检索最新文献以获取准确信息。
**Background of PGM5 Recombinant Protein**
Phosphoglucomutase 5 (PGM5) is a member of the phosphoglucomutase (PGM) enzyme family, traditionally known for its role in carbohydrate metabolism by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. However, PGM5 has distinct characteristics compared to other isoforms (e.g., PGM1-4). Unlike its cytoplasmic counterparts, PGM5 is primarily associated with the cytoskeleton and extracellular matrix (ECM), suggesting roles beyond metabolic regulation. It is expressed in smooth muscle and endothelial cells, where it interacts with cytoskeletal proteins like actin, influencing cell adhesion, motility, and mechanical stability.
The recombinant PGM5 protein is engineered through molecular cloning, often expressed in *E. coli* or mammalian cell systems* to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity, bioactive PGM5 for research applications. Recombinant PGM5 enables detailed study of its non-canonical functions, particularly its involvement in tissue remodeling, fibrosis, and vascular homeostasis.
Emerging studies link PGM5 dysregulation to pathological conditions. For instance, reduced PGM5 expression is observed in cancers (e.g., colorectal, lung) and correlates with tumor progression, metastasis, and poor prognosis. It is hypothesized to act as a tumor suppressor by modulating ECM integrity and cell-matrix signaling. Similarly, PGM5 variants are implicated in cardiovascular diseases, including aortic aneurysms, potentially due to altered smooth muscle cell contractility.
Research on recombinant PGM5 also explores its diagnostic or therapeutic potential. Its ECM-associated activity makes it a candidate biomarker for fibrosis or vascular dysfunction. Furthermore, structural analysis of recombinant PGM5 (e.g., via X-ray crystallography) could reveal novel binding sites for drug targeting.
In summary, recombinant PGM5 serves as a critical tool to unravel the dual metabolic and structural roles of this protein, bridging gaps between basic biochemistry and clinical applications in fibrosis, cancer, and cardiovascular disorders.
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