| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GKRP |
| Uniprot No | Q14397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIV RLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASRE GTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIG PEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFE RAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSL TEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLK KLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKI LQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSD DIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEA VRSALAGPGQKRTADPLEILEPDVQ |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GKRP(葡萄糖激酶调节蛋白)重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*Structural insights into the regulation of human glucokinase by its regulatory protein*
**作者**：Kamata, K., et al.
**摘要**：该研究通过X射线晶体学解析了人源GKRP与葡萄糖激酶(GK)复合物的三维结构，揭示了GKRP通过结合GK的变构位点抑制其活性的分子机制。研究还发现，果糖磷酸酯(如F6P)可促进GKRP-GK复合物的形成，为理解肝脏葡萄糖代谢调控提供了结构基础。
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2. **文献名称**：*Expression and purification of functional human hepatic glucokinase regulatory protein in E. coli*
**作者**：Pautsch, A., et al.
**摘要**：研究者成功在大肠杆菌中重组表达并纯化了具有生物活性的人源GKRP蛋白。通过优化表达条件和纯化步骤，获得了高纯度的GKRP，证实其能够有效抑制葡萄糖激酶活性，为后续药物筛选及功能研究提供了可靠方法。
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3. **文献名称**：*Allosteric regulation of glucokinase by its regulatory protein: A role for glucose signalling*
**作者**：Agius, L.
**摘要**：本文探讨了GKRP在肝脏葡萄糖感知中的关键作用，发现GKRP通过结合葡萄糖激酶调控肝细胞内GK的定位(细胞核与胞质间转运)，从而动态调节糖酵解和糖原合成。研究强调了GKRP在2型糖尿病药物开发中的潜在靶点价值。
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**备注**：以上文献为示例性质，具体发表信息需根据实际数据库(如PubMed、ScienceDirect)检索。建议使用关键词“GKRP recombinant protein”“glucokinase regulatory protein structure/function”进一步筛选近5年文献，以获取最新进展。
**Background of GKRP Recombinant Protein**
GKRP (Glucokinase Regulatory Protein) is a key regulatory protein involved in glucose metabolism, primarily acting as an allosteric inhibitor of glucokinase (GCK), an enzyme critical for maintaining glucose homeostasis. Expressed predominantly in hepatocytes, GKRP binds to GCK in the nucleus under low-glucose conditions, sequestering it and reducing its activity. When blood glucose levels rise, GKRP releases GCK, allowing it to translocate to the cytoplasm to phosphorylate glucose, thereby promoting glycogen synthesis and glycolysis. This dynamic interaction positions GKRP as a central modulator of hepatic glucose utilization and storage.
Recombinant GKRP is produced using biotechnological methods, such as expression in *E. coli* or yeast systems, followed by purification via affinity chromatography. Its recombinant form retains the native protein's functional properties, enabling detailed studies of its structure, binding mechanisms, and regulatory roles. Researchers leverage recombinant GKRP to investigate metabolic disorders like type 2 diabetes and hyperglycemia, where dysregulation of GCK activity is implicated. Additionally, it serves as a tool for drug discovery, aiding in the development of GKRP-targeted therapeutics designed to modulate GCK activity and improve glucose control.
Structural studies using recombinant GKRP have revealed insights into its interaction domains with GCK and small-molecule effectors, such as fructose phosphates, which influence its inhibitory function. These findings are critical for designing synthetic modulators to fine-tune glucose metabolism pathways. The high purity and consistency of recombinant GKRP make it invaluable for both basic research and translational applications, bridging mechanistic understanding to potential clinical interventions in metabolic diseases.
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