| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EMC8 |
| Uniprot No | O43402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAEKQKPRKEHLPLGGPGAHHTLFVDCIPLFHGTLALAPMLEVALTLIDSWCKDHSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDTALIMVDNTKFTMDCVAPTIHVYEHHENRWRCRDPHHDYCEDWPEAQRISASLLDSRSYETLVDFDNHLDDIRNDWTNPEINKAVLHLC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EMC8重组蛋白的3篇参考文献摘要概述：
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1. **文献名称**: *EMC8调控内质网相关降解途径的分子机制研究*
**作者**: Li X, Zhang Y, et al.
**摘要**: 研究揭示了EMC8作为内质网膜复合物(EMC)亚基，通过与Hrd1泛素连接酶互作，参与错误折叠蛋白的降解。重组EMC8蛋白实验表明其通过稳定Hrd1复合物结构促进内质网相关降解(ERAD)过程。
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2. **文献名称**: *EMC8重组蛋白在肝癌细胞侵袭中的作用*
**作者**: Wang H, Chen J, et al.
**摘要**: 研究发现EMC8在肝癌组织中高表达，重组EMC8蛋白过表达可激活MMP2信号通路，增强肝癌细胞迁移和侵袭能力，提示其作为潜在治疗靶点的可能性。
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3. **文献名称**: *Structural insights into the EMC complex and its role in membrane protein biogenesis*
**作者**: O'Donnell JP, Phillips BP, et al.
**摘要**: 通过冷冻电镜解析EMC复合物(含EMC8亚基)的三维结构，发现其作为分子伴侣辅助跨膜蛋白正确折叠。重组EMC8蛋白功能实验证实其在维持内质网稳态中的关键作用。
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注：以上文献为虚拟概括示例，实际研究需查询PubMed、Web of Science等数据库获取真实文献(如涉及EMC复合物或EMC8的论文，如Nature 2019年关于EMC结构的文章)。
**Background of EMC8 Recombinant Protein**
The endoplasmic reticulum membrane protein complex subunit 8 (EMC8) is a conserved component of the EMC, a multi-subunit complex localized to the endoplasmic reticulum (ER). The EMC plays a critical role in membrane protein biogenesis, particularly in the insertion and folding of transmembrane domain-containing proteins. EMC8. along with other EMC subunits, is implicated in maintaining ER homeostasis by facilitating the proper assembly of nascent membrane proteins, thereby reducing misfolding and ER stress.
Studies suggest that EMC8 contributes to ER-associated degradation (ERAD) pathways, aiding in the recognition and clearance of aberrant proteins. Its dysfunction has been linked to disrupted protein quality control, which is associated with neurodegenerative diseases, cancer, and other pathologies. Recombinant EMC8 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables researchers to study its structural and functional roles *in vitro*. This engineered protein retains key biochemical properties, allowing investigations into its interactions with other EMC subunits, client proteins, or chaperones.
EMC8 recombinant protein is also utilized in structural studies (e.g., crystallography or cryo-EM) to decipher the EMC's architecture and mechanism. Furthermore, it serves as a tool for screening potential modulators of ER stress pathways or therapeutic agents targeting EMC8-related disorders. Recent research highlights its involvement in viral replication cycles, as some pathogens exploit the EMC for proper viral protein folding, suggesting EMC8 as a potential antiviral target.
Overall, EMC8 recombinant protein provides a versatile platform for unraveling ER biology, disease mechanisms, and therapeutic strategies tied to protein homeostasis.
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