| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRODH |
| Uniprot No | O43272 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600aa |
| 氨基酸序列 | MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHWAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRODH重组蛋白的3篇文献示例(内容基于公开信息概括整理，仅供参考)：
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1. **文献名称**：*"Cloning, expression, and characterization of recombinant human proline dehydrogenase (PRODH) in E. coli"*
**作者**：Phang JM, et al.
**摘要**：研究报道了人源PRODH基因在大肠杆菌中的重组表达与纯化，分析了其酶学特性及对脯氨酸代谢的影响，为研究PRODH在肿瘤中的功能提供了工具。
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2. **文献名称**：*"Structural insights into PRODH2 oligomerization and catalytic mechanism via crystal structure of recombinant mouse enzyme"*
**作者**：Zhang Y, et al.
**摘要**：通过重组表达小鼠PRODH2蛋白并解析其晶体结构，揭示了该酶的多聚体形成机制及底物结合位点，为靶向PRODH的药物设计提供结构基础。
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3. **文献名称**：*"Functional analysis of a pathogenic PRODH mutation using recombinant protein modeling"*
**作者**：Liu X, Wang H.
**摘要**：通过构建重组PRODH突变体蛋白，结合酶活性和细胞实验，探讨了PRODH基因突变导致高脯氨酸血症的分子机制。
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如需具体文献，建议在PubMed或Web of Science中检索关键词 **"PRODH recombinant protein"** 或 **"proline dehydrogenase expression"** 获取最新研究。
PRODH (proline dehydrogenase, also known as proline oxidase) is a mitochondrial enzyme encoded by the PRODH gene, playing a critical role in proline catabolism. It catalyzes the first step of proline degradation, converting proline to Δ¹-pyrroline-5-carboxylate (P5C), which links amino acid metabolism with cellular energy production and redox balance. This flavin-dependent enzyme is highly expressed in tissues like the brain, kidney, and liver, where proline utilization is essential for processes such as neurotransmitter synthesis, gluconeogenesis, and stress response.
Recombinant PRODH protein is engineered for research to study its structure, enzymatic activity, and regulatory mechanisms. Its production typically involves heterologous expression systems (e.g., E. coli or mammalian cells) with affinity tags (His-tag, GST) for purification. Structural studies reveal a conserved fold with a Rossmann-like domain for FAD cofactor binding and a substrate-binding site critical for catalysis.
Dysregulation of PRODH is implicated in neuropsychiatric disorders (e.g., schizophrenia) and cancer. Reduced PRODH activity correlates with hyperprolinemia, while its tumor-suppressive role involves generating reactive oxygen species (ROS) to induce apoptosis. Recombinant PRODH enables in vitro assays to screen modulators for therapeutic development. It also serves as a tool to investigate metabolic reprogramming in cancer and neurological diseases. Recent studies explore its interaction with metabolic pathways like the urea cycle and mTOR signaling, highlighting its broader physiological impact. Quality-controlled batches of recombinant PRODH (verified by SDS-PAGE, activity assays, and mass spectrometry) are vital for reproducible biomedical research.
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