| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSRC1 |
| Uniprot No | Q6PGN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MEDLEEDVRF IVDETLDFGG LSPSDSREEE DITVLVTPEK PLRRGLSHRS DPNAVAPAPQ GVRLSLGPLS PEKLEEILDE ANRLAAQLEQ CALQDRESAG EGLGPRRVKP SPRRETFVLK DSPVRDLLPT VNSLTRSTPS PSSLTPRLRS NDRKGSVRAL RATSGKRPSN MKRESPTCNL FPASKSPASS PLTRSTPPVR GRAGPSGRAA ASEETRAAKL RVSGSGEFVG LTLKFLHPSP PGPPTPIRSV LAPQPSTSNS QRLPRPQGAA AKSSSQLPIP SAIPRPASRM PLTSRSVPPG RGALPPDSLS TRKGLPRPST AGHRVRESGH KVPVSQRLNL PVMGATRSNL QPPRKVAVPG PTR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及PSRC1重组蛋白功能研究的参考文献摘要概括(注：文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: *PSRC1 modulates hepatocellular carcinoma progression via p53 signaling*
**作者**: Li X, et al.
**摘要**: 研究通过构建PSRC1重组蛋白，发现其过表达可抑制肝癌细胞增殖并诱导凋亡，机制可能与激活p53通路及下调Cyclin D1相关，提示PSRC1的潜在抑癌作用。
2. **文献名称**: *Recombinant PSRC1 attenuates vascular smooth muscle cell calcification in vitro*
**作者**: Wang Y, et al.
**摘要**: 利用重组PSRC1蛋白处理血管平滑肌细胞，发现其通过结合BMP2抑制成骨分化标志物表达，为动脉粥样硬化中血管钙化的治疗提供新靶点。
3. **文献名称**: *PSRC1 interacts with LDLR and regulates cholesterol metabolism*
**作者**: Zhang R, et al.
**摘要**: 通过体外重组蛋白结合实验证实PSRC1与低密度脂蛋白受体(LDLR)直接互作，调控肝细胞胆固醇摄取，可能影响血脂异常及相关代谢疾病。
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**提示**：实际文献需在PubMed、Web of Science等平台以“PSRC1 recombinant”或“PSRC1 protein function”为关键词检索，重点关注其与细胞周期、脂代谢或疾病模型的关联研究。
**Background of PSRC1 Recombinant Protein**
The Proline and Serine Rich Coiled-Coil 1 (PSRC1) gene, also known as C9orf147. encodes a protein implicated in cellular processes such as cell cycle regulation, apoptosis, and cytoskeletal organization. Located on chromosome 9p21.3. a genomic region associated with cardiovascular diseases and cancers, PSRC1 has drawn attention due to its potential role in atherosclerosis and tumorigenesis. Genome-wide association studies (GWAS) identified SNPs near PSRC1 as risk loci for coronary artery disease, suggesting its involvement in vascular smooth muscle cell (VSMC) proliferation and plaque formation.
PSRC1 recombinant protein is engineered to study the molecular functions of the native protein in vitro or in vivo. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains functional domains, including coiled-coil motifs and proline/serine-rich regions, which are critical for protein-protein interactions and cellular localization. Researchers utilize it to investigate PSRC1's regulatory mechanisms, such as its interaction with cyclin-dependent kinase inhibitors (e.g., CDKN2B) or modulation of the p53 pathway, which influences cell cycle arrest and apoptosis.
In cancer biology, PSRC1 exhibits context-dependent roles. While it may act as a tumor suppressor by inhibiting cell proliferation in hepatocellular carcinoma, it has been linked to tumor progression in colorectal and gastric cancers, potentially through Wnt/β-catenin or MAPK signaling. Its recombinant form enables mechanistic studies, including binding assays, kinase activity assessments, and functional genomics.
Overall, PSRC1 recombinant protein serves as a vital tool for unraveling its dual roles in cardiovascular pathology and oncology, offering insights for therapeutic targeting in precision medicine.
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