| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LEPREL1 |
| Uniprot No | Q8IVL5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-708aa |
| 氨基酸序列 | GPPDSP RRELELEPGP LQPFDLLYAS GAAAYYSGDY ERAVRDLEAA LRSHRRLREI RTRCARHCAA RHPLPPPPPG EGPGAELPLF RSLLGRARCY RSCETQRLGG PASRHRVSED VRSDFQRRVP YNYLQRAYIK LNQLEKAVEA AHTFFVANPE HMEMQQNIEN YRATAGVEAL QLVDREAKPH MESYNAGVKH YEADDFEMAI RHFEQALREY FVEDTECRTL CEGPQRFEEY EYLGYKAGLY EAIADHYMQV LVCQHECVRE LATRPGRLSP IENFLPLHYD YLQFAYYRVG EYVKALECAK AYLLCHPDDE DVLDNVDYYE SLLDDSIDPA SIEAREDLTM FVKRHKLESE LIKSAAEGLG FSYTEPNYWI RYGGRQDENR VPSGVNVEGA EVHGFSMGKK LSPKIDRDLR EGGPLLYENI TFVYNSEQLN GTQRVLLDNV LSEEQCRELH SVASGIMLVG DGYRGKTSPH TPNEKFEGAT VLKALKSGYE GRVPLKSARL FYDISEKARR IVESYFMLNS TLYFSYTHMV CRTALSGQQD RRNDLSHPIH ADNCLLDPEA NECWKEPPAY TFRDYSALLY MNDDFEGGEF IFTEMDAKTV TASIKPKCGR MISFSSGGEN PHGVKAVTKG KRCAVALWFT LDPLYRELER IQADEVIAIL DQEQQGKHEL NINPKDEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LEPREL1重组蛋白的虚构参考文献示例，内容基于该蛋白的典型研究方向：
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1. **文献名称**：*Expression and Enzymatic Characterization of Recombinant Human LEPREL1 Prolyl 3-Hydroxylase*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人源LEPREL1蛋白，并通过体外酶活实验证实其对胶原肽链中特定脯氨酸残基的3-羟基化修饰功能，揭示了其依赖α-酮戊二酸和Fe²⁺的催化机制。
2. **文献名称**：*Crystal Structure of LEPREL1 Reveals Substrate Binding Specificity*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了LEPREL1重组蛋白的三维结构，发现其底物结合口袋的独特构象，解释了其对胶原IV型链的特异性识别，为靶向胶原代谢疾病的药物设计提供了结构基础。
3. **文献名称**：*LEPREL1 Knockdown Impairs Collagen Maturation and Promotes Tumor Metastasis*
**作者**：Chen L, et al.
**摘要**：在乳腺癌模型中，LEPREL1重组蛋白的功能缺失导致胶原纤维结构异常，增强癌细胞侵袭能力，表明其通过调控细胞外基质完整性抑制肿瘤转移。
4. **文献名称**：*LEPREL1 Mutations Alter Collagen Hydroxylation in Osteogenesis Imperfecta*
**作者**：Müller R, et al.
**摘要**：在成骨不全症患者中发现LEPREL1基因突变，重组蛋白实验显示突变体酶活性显著降低，导致胶原交联缺陷，提示其在骨骼发育中的关键作用。
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**注**：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
LEPREL1 (Leprecan Homolog 1), also known as Prolyl 3-Hydroxylase 2 (P3H2), is a member of the lysyl hydroxylase family that plays a critical role in collagen biosynthesis. This enzyme catalyzes the post-translational hydroxylation of specific proline and lysine residues in collagen chains, a modification essential for stabilizing collagen triple helices and ensuring proper extracellular matrix (ECM) assembly. LEPREL1 is particularly notable for its 3-hydroxylation activity on the Pro986 residue in the α1 chain of type I collagen, a key step in collagen maturation. Dysregulation of LEPREL1 has been implicated in connective tissue disorders, fibrosis, and cancer metastasis due to its influence on ECM integrity and cell signaling.
Recombinant LEPREL1 protein is engineered to study its enzymatic mechanisms and interactions. Typically produced in mammalian or insect cell systems to ensure proper folding and post-translational modifications, the recombinant protein retains hydroxylase activity and can be used in vitro to investigate collagen modification pathways. Researchers employ it to model diseases like osteogenesis imperfecta, where collagen hydroxylation defects are pathological hallmarks. Additionally, it serves as a tool for drug screening targeting collagen-related disorders or fibrosis. Structural studies using recombinant LEPREL1 have revealed insights into its substrate-binding domains and catalytic sites, distinguishing it from other isoforms like P3H1/P3H3. Despite its functional significance, LEPREL1's regulatory networks and tissue-specific roles remain partially unexplored, driving ongoing research to clarify its contributions to ECM dynamics and therapeutic potential.
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