| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTTN |
| Uniprot No | Q14247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-513aa |
| 氨基酸序列 | MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQ EHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAV GHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHAS QKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYG IDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGW DHQEKLQLHESQKDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKT VPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEA RRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPV SGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYD EYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGR YGLFPANYVELRQ |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTTN(Cortactin)重组蛋白的3篇参考文献的简要信息(注：文献为示例性内容，实际引用需核实具体来源)：
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1. **文献名称**：*"Recombinant Cortactin (CTTN) promotes actin polymerization and enhances cell migration in vitro"*
**作者**：Smith J, et al.
**摘要**：研究通过表达并纯化重组人源CTTN蛋白，证实其可通过激活Arp2/3复合体促进肌动蛋白聚合，增强肿瘤细胞的迁移能力，为CTTN在癌症转移中的作用提供机制证据。
2. **文献名称**：*"Functional analysis of CTTN phosphorylation sites using recombinant mutants in invadopodia formation"*
**作者**：Li Y, et al.
**摘要**：构建了不同磷酸化位点突变的重组CTTN蛋白，发现Tyr421磷酸化对侵袭性伪足(invadopodia)的形成及细胞外基质降解至关重要，揭示CTTN在肿瘤侵袭中的调控网络。
3. **文献名称**：*"Purification and structural characterization of recombinant CTTN for crystallography studies"*
**作者**：Wang H, et al.
**摘要**：优化了大肠杆菌表达系统的高纯度CTTN重组蛋白制备方法，并通过X射线晶体学解析其N端结构域的三维构象，为靶向CTTN的药物设计提供结构基础。
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**提示**：实际引用时建议通过PubMed或Web of Science检索关键词(如“CTTN recombinant protein”、“Cortactin purification”)获取最新研究，并优先选择高影响因子期刊(如*JBC*、*Cell Reports*等)的文献。
Cortactin (CTTN), encoded by the *CTTN* gene, is a dynamic cytoskeletal protein regulating cell motility, adhesion, and membrane remodeling. It binds actin filaments and activates the Arp2/3 complex to promote branched actin network formation, critical for processes like lamellipodia protrusion, endocytosis, and invadopodia-mediated extracellular matrix degradation. Structurally, cortactin contains an N-terminal acidic domain, a central tandem repeat region (actin-binding sites), and a C-terminal SH3 domain facilitating protein-protein interactions.
Recombinant CTTN protein, produced via bacterial (e.g., *E. coli*) or mammalian expression systems, retains these functional domains. Its production often includes affinity tags (e.g., His-tag) for purification. Researchers utilize recombinant CTTN to study its role in cancer metastasis, as its overexpression correlates with tumor invasion and poor prognosis. It also serves as a tool to investigate signaling pathways (e.g., EGFR, Src kinase) regulating cytoskeletal dynamics.
In neurobiology, cortactin is implicated in synaptic plasticity and dendritic spine maturation. Recombinant protein studies help dissect its interplay with postsynaptic density proteins. Additionally, CTTN variants or phosphorylation-mimetic mutants are engineered to explore post-translational modifications (e.g., tyrosine phosphorylation by Src) that modulate its activity.
Applications extend to drug discovery, where recombinant CTTN aids in screening inhibitors targeting actin-driven invasion in cancers. Its interaction with therapeutic targets (e.g., HER2. FAK) underscores its biomedical relevance. Overall, recombinant CTTN remains pivotal for elucidating cytoskeletal regulation mechanisms and developing anti-metastatic strategies.
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