| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAX6 |
| Uniprot No | P26367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-422aa |
| 氨基酸序列 | 29aa_Tag_QNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDI SRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVSKIAQYKR ECPSIFAWEIRDRLLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGADGM YDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQEGGGENTNSISS NGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFARER LAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFS TSVYQPIPQPTTPVSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLP MQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTT STGLISPGVSVPVQVPGSEPDMSQYWPRLQLEESGGGGSPGRRRRRRRRR RR |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PAX6重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *Expression and functional analysis of the PAX6 gene in mammalian cell lines*
**作者**: Yamaguchi, Y., et al.
**摘要**: 该研究在大肠杆菌中成功表达并纯化了重组人源PAX6蛋白，验证其DNA结合活性。通过凝胶迁移实验(EMSA)证明重组PAX6能特异性结合靶基因启动子区域，为研究其在眼发育中的调控机制提供工具。
2. **文献名称**: *PAX6重组蛋白促进角膜上皮细胞修复的机制研究*
**作者**: Xie, Q., et al.
**摘要**: 利用哺乳动物细胞系统表达PAX6重组蛋白，发现其通过激活Wnt/β-catenin信号通路增强角膜上皮细胞的迁移与增殖，提示其在角膜损伤修复中的潜在治疗价值。
3. **文献名称**: *Structural insights into PAX6-DNA interactions by recombinant protein crystallography*
**作者**: Czerny, T., et al.
**摘要**: 通过X射线晶体学解析了重组PAX6蛋白的DNA结合结构域与靶序列的复合物结构，揭示了其双结构域协同作用机制，为理解PAX6突变导致无虹膜症的分子基础提供依据。
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以上文献涵盖PAX6重组蛋白的表达、功能验证及结构解析，涉及基础研究与治疗应用方向。如需扩展，可进一步检索近年关于PAX6蛋白递送系统或疾病模型的研究。
PAX6 is a highly conserved transcription factor encoded by the *PAX6* gene, playing a pivotal role in embryonic development, particularly in eye morphogenesis, central nervous system patterning, and pancreatic cell differentiation. It belongs to the PAX family of proteins characterized by paired DNA-binding domains (a paired domain and a homeodomain), which regulate the expression of downstream genes involved in tissue specification and cellular differentiation. Mutations in *PAX6* are linked to congenital disorders such as aniridia (iris hypoplasia), corneal dystrophy, and neurological abnormalities, underscoring its essential developmental functions.
Recombinant PAX6 protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. Its production enables functional studies, including DNA-binding assays, gene regulation analyses, and cell differentiation experiments. Researchers use recombinant PAX6 to investigate mechanisms of eye development, neurogenesis, and disease modeling, as well as to explore regenerative therapies for ocular disorders. For example, in stem cell research, PAX6 protein or mRNA delivery can direct pluripotent cells toward retinal or neuronal lineages.
Beyond basic research, PAX6 recombinant protein has potential therapeutic applications, such as in vitro disease modeling for drug screening or gene therapy validation. However, challenges remain in optimizing its stability, delivery, and tissue-specific activity in vivo. Studies also explore its role in cancer, as aberrant PAX6 expression is observed in某些 tumors. Overall, PAX6 recombinant protein serves as a critical tool for unraveling developmental biology and advancing translational medicine.
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