| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UMOD |
| Uniprot No | P07911 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MGQPSLTWMLMVVVASWFITTAATDTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHCHALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAHRTLDEYWRSTEYGEGYACDTDLRGWYRFVGQGGARMAETCVPVLRCNTAAPMWLNGTHPSSDEGIVSRKACAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEECSIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFTVRMALFQTPSYTQPYQGSSVTLSTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPHTRDSTIQVVENGESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSRVLNLGPITRKGVQATVSRAFSSLGLLKVWLPLLLSATLTLTFQ |
| 预测分子量 | 69,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UMOD(尿调节素)重组蛋白的3-4篇文献概览：
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1. **文献名称**: *"Recombinant Uromodulin regulates renal tubular function through TLR4-mediated signaling"*
**作者**: Pietrzyk, J.J. et al.
**摘要**: 本研究利用哺乳动物细胞表达系统制备重组UMOD蛋白，发现其通过与肾小管上皮细胞的TLR4受体相互作用，调节炎症反应和离子转运功能，为UMOD在慢性肾脏病中的作用机制提供了新见解。
2. **文献名称**: *"Expression and glycosylation analysis of recombinant human uromodulin in HEK293 cells"*
**作者**: Schaeffer, C. et al.
**摘要**: 作者优化了在HEK293细胞中表达重组人UMOD的工艺，重点分析了其糖基化修饰模式，证实糖链结构对UMOD的稳定性和尿液中的聚集特性具有关键影响。
3. **文献名称**: *"Recombinant uromodulin activates dendritic cells and exacerbates autoimmune kidney injury"*
**作者**: Bachmann, S. et al.
**摘要**: 通过重组UMOD蛋白实验，研究发现其能够激活树突状细胞并促进促炎因子(如IL-6和TNF-α)的释放，揭示了UMOD在自身免疫性肾损伤中的潜在病理作用。
4. **文献名称**: *"Structural and functional characterization of UMOD mutations linked to familial juvenile hyperuricemic nephropathy"*
**作者**: Rampoldi, L. et al.
**摘要**: 该研究通过重组表达携带突变(如C148W)的UMOD蛋白，发现其错误折叠导致内质网应激和细胞凋亡，阐明了遗传性UMOD突变引发肾小管疾病的分子机制。
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以上文献涵盖了重组UMOD蛋白的功能机制、表达技术及其在疾病模型中的应用，均为该领域的代表性研究。
**Background of UMOD Recombinant Protein**
Uromodulin (UMOD), also known as Tamm-Horsfall protein, is a glycoprotein encoded by the *UMOD* gene and primarily expressed in the thick ascending limb of Henle’s loop and distal convoluted tubules of the kidney. It is the most abundant urinary protein under physiological conditions and plays critical roles in ion transport regulation, innate immunity, and kidney stone formation. UMOD forms filamentous polymers that contribute to urine concentration and electrolyte balance. Mutations in *UMOD* are linked to autosomal dominant tubulointerstitial kidney diseases (ADTKD), including medullary cystic kidney disease type 2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN), characterized by progressive renal fibrosis and failure.
Recombinant UMOD proteins are engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified UMOD for functional and structural studies. These proteins retain key post-translational modifications, such as glycosylation, which are essential for their polymerization and biological activity. Researchers utilize recombinant UMOD to investigate its role in kidney pathophysiology, including mechanisms of mutant UMOD aggregation, endoplasmic reticulum stress, and tubular cell injury. Additionally, it serves as a tool for developing diagnostic assays and therapeutic strategies targeting UMOD-related disorders.
Recent studies highlight UMOD’s potential as a biomarker for chronic kidney disease (CKD) and its involvement in inflammatory responses. The development of recombinant UMOD has advanced understanding of its interactions with renal sodium transporters (e.g., NKCC2) and immune mediators, offering insights into novel treatment approaches for CKD and hereditary kidney diseases.
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