| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | URAT1 |
| Uniprot No | Q96S37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF |
| 预测分子量 | 59,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于URAT1重组蛋白的3篇代表性文献及其摘要内容概括：
1. **文献名称**：*Molecular Identification of a Renal Urate Anion Exchanger that Regulates Blood Urate Levels*
**作者**：Enomoto A, et al.
**摘要**：首次克隆并鉴定了人类URAT1基因，在非洲爪蟾卵母细胞中重组表达，证实其作为尿酸/阴离子交换器的功能，并发现其突变与肾性低尿酸血症相关。
2. **文献名称**：*Functional characterization of human URAT1 recombinant protein: Transport properties and inhibitor screening*
**作者**：Hagos Y, et al.
**摘要**：在HEK293细胞中重组表达URAT1蛋白，系统分析其尿酸转运动力学及pH依赖性，并验证苯溴马隆等抑制剂的靶向作用机制。
3. **文献名称**：*Structural insights into human URAT1 by cryo-EM and its inhibition mechanism*
**作者**：Zhou Y, et al.
**摘要**：利用冷冻电镜解析URAT1重组蛋白的高分辨率结构，揭示其底物结合口袋的构象变化，为设计新型痛风治疗药物提供结构基础。
(注：以上文献为示例，实际引用时建议通过PubMed或SciHub核对具体信息。)
**Background of URAT1 Recombinant Protein**
URAT1 (Urate Transporter 1), encoded by the *SLC22A12* gene, is a key membrane protein predominantly expressed in the apical membrane of renal proximal tubule cells. It belongs to the solute carrier family 22 (SLC22) and functions as a urate/anion exchanger, playing a critical role in regulating uric acid reabsorption in the kidneys. Dysregulation of URAT1 is closely associated with hyperuricemia, gout, and related metabolic disorders, making it a therapeutic target for antihyperuricemic drugs like benzbromarone and lesinurad.
Recombinant URAT1 protein is artificially produced using molecular cloning techniques, often expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and membrane localization. This engineered protein retains the functional characteristics of native URAT1. enabling in vitro studies to investigate its transport mechanisms, ligand interactions, and inhibition kinetics. Researchers utilize URAT1 recombinant protein to screen potential inhibitors, analyze structure-function relationships, and model pathological conditions like hereditary hypouricemia caused by *SLC22A12* mutations.
The development of URAT1 recombinant protein has advanced drug discovery, particularly for gout treatment, by providing a reliable platform for high-throughput assays and structural studies (e.g., cryo-EM or X-ray crystallography). Additionally, it aids in understanding how genetic variations or drug interactions influence urate homeostasis, offering insights into personalized therapeutic strategies. Its application extends to toxicology studies, assessing drug-induced nephrotoxicity linked to URAT1 dysfunction. Overall, URAT1 recombinant protein serves as a vital tool in both basic research and clinical translation for metabolic and renal diseases.
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