| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPS8 |
| Uniprot No | Q99627 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPVAVMAESAFSFKKLLDQCENQELEAPGG IATPPVYGQLLALYLLHNDMNNARYLWKRIPPAIKSANSELGGIWSVGQR IWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIA DDFAAFVGLPVEEAVKGILEQGWQADSTTRMVLPRKPVAGALDVSFNKFI PLSEPAPVPPIPNEQQLARLTDYVAFLEN |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COPS8重组蛋白的示例参考文献(注：文献为示例，非真实存在)：
---
1. **文献名称**：*Structural Insights into COPS8 Recombinant Protein and Its Role in the COP9 Signalosome*
**作者**：Smith A, et al.
**摘要**：通过大肠杆菌系统重组表达COPS8蛋白，结合X射线晶体学分析其三维结构，揭示了其与COP9复合体其他亚基(如COPS5)相互作用的关键结构域，并证明COPS8在调控泛素连接酶活性中的必要性。
2. **文献名称**：*Functional Characterization of Recombinant COPS8 in Cancer Cell Models*
**作者**：Lee B, et al.
**摘要**：利用哺乳动物细胞表达重组COPS8.发现其过表达显著抑制肿瘤细胞增殖，并通过调控细胞周期蛋白p27的稳定性影响细胞周期进程，提示COPS8可能作为潜在的癌症治疗靶点。
3. **文献名称**：*Recombinant COPS8 Facilitates In Vitro Assembly of the COP9 Complex*
**作者**：Garcia C, et al.
**摘要**：通过昆虫细胞系统表达并纯化重组COPS8.结合体外重组实验证实其在COP9复合体组装中的核心作用，并证明其缺失会导致复合体功能异常，影响去泛素化酶活性。
4. **文献名称**：*COPS8 Recombinant Protein Interacts with Cullin Family Proteins: Implications for Ubiquitination Pathways*
**作者**：Zhang D, et al.
**摘要**：利用重组COPS8进行体外结合实验，发现其与CUL1和CUL4等Cullin家族蛋白直接互作，揭示了COPS8通过调控Cullin-RING泛素连接酶(CRLs)活性参与蛋白质降解通路的分子机制。
---
**备注**：以上文献为基于COPS8已知功能的模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of COPS8 Recombinant Protein**
The COP9 signalosome (CSN) is a highly conserved multi-protein complex involved in regulating ubiquitin-proteasome-mediated protein degradation, a critical process for cellular homeostasis. Among its eight subunits (CSN1 to CSN8), **COPS8** (CSN subunit 8) plays a pivotal role in maintaining the structural integrity and enzymatic activity of the complex. The CSN complex primarily regulates the activity of cullin-RING ubiquitin ligases (CRLs), which tag proteins for proteasomal degradation. COPS8. though the smallest subunit, is essential for stabilizing the interaction between CSN and CRLs, thereby modulating substrate specificity and degradation efficiency.
Recombinant COPS8 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems, to ensure proper folding and post-translational modifications. Its production enables detailed biochemical and functional studies, such as elucidating interactions within the CSN complex or dissecting its role in cellular pathways like DNA repair, cell cycle progression, and stress responses. Dysregulation of COPS8 has been linked to diseases, including cancers and neurodegenerative disorders, highlighting its therapeutic relevance.
Recent studies also suggest COPS8's involvement in immune regulation and developmental processes, making it a target for drug discovery. By leveraging recombinant COPS8. researchers aim to uncover mechanisms underlying CSN-associated pathologies and develop strategies to modulate CRL activity for precision medicine applications.
×
