| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UPK1A |
| Uniprot No | O00322 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MASAAAAEAEKGSPVVVGLLVVGNIIILLSGLSLFAETIWVTADQYRVYPLMGVSGKDDVFAGAWIAIFCGFSFFMVASFGVGAALCRRRSMVLTYLVLMLIVYIFECASCITSYTHRDYMVSNPSLITKQMLTFYSADTDQGQELTRLWDRVMIEQECCGTSGPMDWVNFTSAFRAATPEVVFPWPPLCCRRTGNFIPLNEEGCRLGHMDYLFTKGCFEHIGHAIDSYTWGISWFGFAILMWTLPVMLIAMYFYTML |
| 预测分子量 | 28,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UPK1A重组蛋白的示例参考文献(部分为假设性示例，仅供参考)：
1. **"Recombinant expression and functional characterization of human UPK1A in bladder cancer models"**
*Authors: Zhang L, Wang Y, Chen H*
**摘要**: 本研究通过哺乳动物细胞表达系统成功制备了重组UPK1A蛋白，并发现其在膀胱癌细胞中通过调控细胞间粘附影响肿瘤迁移能力。实验表明UPK1A过表达可抑制EMT过程。
2. **"Structural analysis of Uroplakin 1A using cryo-EM reveals its role in urothelial barrier formation"**
*Authors: Tanaka K, et al.*
**摘要**: 利用冷冻电镜解析了重组UPK1A蛋白的跨膜结构域三维构象，揭示了其与UPK2形成异源二聚体调控尿路上皮屏障功能的分子机制。
3. **"Development of a UPK1A-based urine biomarker assay for urothelial carcinoma detection"**
*Authors: Müller R, et al.*
**摘要**: 通过原核表达系统获得高纯度重组UPK1A蛋白，开发出基于ELISA的检测方法，证明尿液中UPK1A异常糖基化可作为膀胱癌诊断的生物标志物。
4. **"Comparative study of UPK1A orthologs in vertebrate urinary tract development"**
*Authors: Smith J, et al.*
**摘要**: 跨物种重组蛋白表达分析显示，斑马鱼和小鼠UPK1A在进化中高度保守，通过CRISPR敲除模型证实其参与胚胎期尿路上皮细胞分化。
注：以上为模拟文献示例，实际文献需通过PubMed/Web of Science等平台检索确认。建议使用关键词"UPK1A recombinant protein"或"Uroplakin 1A expression"进行精准检索。
**Background of UPK1A Recombinant Protein**
UPK1A (Uroplakin 1A) is a transmembrane protein belonging to the uroplakin family, which plays a critical role in maintaining the permeability barrier and mechanical stability of the urothelium, the specialized epithelial layer lining the urinary tract. Uroplakins, including UPK1A, assemble into lipid-raft membrane complexes that form the apical plaques of umbrella cells, contributing to urine-blood barrier integrity and cellular signaling. UPK1A is structurally characterized by four transmembrane domains and forms heterodimers with UPK2. a process essential for trafficking to the apical membrane.
The recombinant UPK1A protein is engineered using molecular cloning techniques, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications and functional folding. This recombinant form retains the native protein’s structural and biochemical properties, enabling studies on its role in urothelial physiology and pathology. Dysregulation of UPK1A has been implicated in bladder disorders, including interstitial cystitis, urinary tract infections, and urothelial carcinoma, where its expression may serve as a diagnostic or prognostic biomarker.
In research, UPK1A recombinant protein is utilized to investigate cell-cell adhesion, membrane dynamics, and signaling pathways. It also aids in developing therapeutic strategies, such as targeting uroplakin-mediated pathways in bladder cancers or engineering biomimetic surfaces for tissue repair. Its application extends to antibody production, drug screening, and structural studies via crystallography or cryo-EM. Overall, UPK1A recombinant protein is a vital tool for advancing understanding of urothelial biology and related diseases.
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