| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UCN2 |
| Uniprot No | Q96RP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 72-112aa |
| 氨基酸序列 | IVLSLDVPIGLLQILLEQARARAAREQATTNARILARVGHC |
| 预测分子量 | 17.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UCN2重组蛋白的3篇代表性文献，涵盖功能、应用及制备研究：
1. **《Recombinant Urocortin 2 Prevents Doxorubicin-induced Cardiotoxicity via AMPK Activation》**
- **作者**: Smith, J.R. et al.
- **摘要**: 研究重组UCN2蛋白通过激活AMPK通路，减轻阿霉素诱导的小鼠心肌细胞凋亡和氧化应激，提出其在化疗心脏保护中的潜在应用。
2. **《Efficient Expression and Purification of Human Urocortin 2 in E. coli for Therapeutic Development》**
- **作者**: Chen, L. & Wang, H.
- **摘要**: 开发了一种基于大肠杆菌的高效重组UCN2表达与纯化系统，优化包涵体复性工艺，获得高纯度活性蛋白，为规模化生产奠定基础。
3. **《Urocortin 2 Regulates Glucose Homeostasis through β-Cell Protection in Diabetic Models》**
- **作者**: Gupta, S. et al.
- **摘要**: 发现重组UCN2可增强糖尿病小鼠胰岛β细胞存活率，改善胰岛素分泌及血糖控制，提示其在糖尿病治疗中的潜在价值。
**备注**：以上文献信息为示例，实际引用需通过PubMed或Web of Science核对真实论文。建议补充关键词(如物种、疾病模型)以获取更精准的文献。
Urocortin 2 (UCN2), a member of the corticotropin-releasing hormone (CRH) neuropeptide family, is a stress-related peptide primarily involved in modulating physiological responses to stress, energy homeostasis, and cardiovascular function. It selectively binds to the type 2 corticotropin-releasing hormone receptor (CRHR2), which is widely expressed in peripheral tissues (e.g., heart, skeletal muscle) and specific brain regions. Unlike UCN1. which activates both CRHR1 and CRHR2. UCN2 exhibits higher specificity for CRHR2. making it a key player in stress adaptation pathways distinct from classical hypothalamic-pituitary-adrenal (HPA) axis activation.
Recombinant UCN2 protein is engineered using genetic cloning techniques, typically expressed in bacterial or mammalian cell systems to ensure proper post-translational modifications. Its mature form consists of 38 amino acids, derived from a precursor protein through enzymatic cleavage. Researchers utilize recombinant UCN2 to study CRHR2-mediated signaling mechanisms, which influence vasodilation, cardiac contractility, and metabolic regulation. Preclinical studies highlight its therapeutic potential in heart failure, hypertension, and metabolic disorders due to its cardioprotective and anti-inflammatory properties. However, challenges remain in optimizing its pharmacokinetic profile and delivery methods for clinical translation. Current investigations also explore its role in appetite regulation and anxiety-related behaviors, expanding its relevance to neuroendocrinology and integrative physiology.
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