| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBP 4 |
| Uniprot No | Q96PP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MGERTLHAAV PTPGYPESES IMMAPICLVE NQEEQLTVNS KALEILDKIS QPVVVVAIVG LYRTGKSYLM NRLAGKRNGF PLGSTVQSET KGIWMWCVPH LSKPNHTLVL LDTEGLGDVE KSNPKNDSWI FALAVLLSSS FVYNSVSTIN HQALEQLHYV TELAELIRAK SCPRPDEAED SSEFASFFPD FIWTVRDFTL ELKLDGNPIT EDEYLENALK LIPGKNPKIQ NSNMPRECIR HFFRKRKCFV FDRPTNDKQY LNHMDEVPEE NLERHFLMQS DNFCSYIFTH AKTKTLREGI IVTGKRLGTL VVTYVDAINS GAVPCLENAV TALAQLENPA AVQRAADHYS QQMAQQLRLP TDTLQELLDV HAACEREAIA VFMEHSFKDE NHEFQKKLVD TIEKKKGDFV LQNEEASAKY CQAELKRLSE HLTESILRGI FSVPGGHNLY LEEKKQVEWD YKLVPRKGVK ANEVLQNFLQ SQVVVEESIL QSDKALTAGE KAIAAERAMK EAAEKEQELL REKQKEQQQM MEAQERSFQE YMAQMEKKLE EERENLLREH ERLLKHKLKV QEEMLKEEFQ KKSEQLNKEI NQLKEKIEST KNEQLRLLKI LDMASNIMIV TLPGASKLLG VGTKYLGSRI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GBP4重组蛋白的3篇参考文献(信息基于公开研究整理，部分内容为模拟概括)：
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1. **文献名称**: *"Recombinant GBP4 Expression and Its Role in Antiviral Immunity"*
**作者**: Smith A, et al.
**摘要**: 研究报道了人源GBP4重组蛋白在大肠杆菌中的高效表达与纯化，证实其通过水解GTP激活免疫信号通路，显著抑制疱疹病毒复制，揭示了GBP4在细胞自主免疫中的直接抗病毒功能。
2. **文献名称**: *"Structural Insights into GBP4 Oligomerization and Bacterial Membrane Targeting"*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组小鼠GBP4的寡聚化结构，发现其N端结构域介导的自组装能力对靶向沙门氏菌膜结构至关重要，为GBP家族蛋白的抗菌机制提供了分子基础。
3. **文献名称**: *"GBP4 Regulates Inflammasome Activation in Macrophages via LPS Sensing"*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组斑马鱼GBP4蛋白进行功能实验，证明其通过结合脂多糖(LPS)增强NLRP3炎症小体活性，提示GBP4在宿主抵御革兰氏阴性菌感染中的调控作用。
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**注**：以上文献信息为示例性概括，具体研究请以实际发表的论文为准。建议通过PubMed或Web of Science以关键词“GBP4 recombinant”“Guanylate-binding protein 4”检索最新文献。
**Background of GBP4 Recombinant Protein**
Guanylate-binding protein 4 (GBP4) is a member of the guanylate-binding protein (GBP) family, a class of interferon (IFN)-inducible GTPases implicated in innate immunity and host defense against intracellular pathogens. GBPs are evolutionarily conserved in vertebrates and play critical roles in combating bacterial, viral, and parasitic infections by targeting microbial membranes, regulating inflammasome activation, and modulating immune signaling pathways. GBP4. specifically, is encoded by the *GBP4* gene in humans and shares structural homology with other GBP family members, including a characteristic N-terminal globular GTPase domain and a C-terminal α-helical region.
Recombinant GBP4 refers to the protein produced artificially using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems. This allows large-scale production of functional GBP4 for research and therapeutic applications. The recombinant form retains the protein’s biochemical properties, such as GTP hydrolysis activity and oligomerization capacity, enabling studies on its role in pathogen resistance, autophagy, and cytokine regulation.
Research highlights GBP4’s involvement in immune responses to intracellular pathogens like *Chlamydia trachomatis* and *Toxoplasma gondii*, where it disrupts pathogen vacuoles or promotes inflammasome-mediated clearance. Dysregulation of GBP4 has also been linked to autoimmune diseases (e.g., lupus) and cancer, suggesting broader implications in inflammation and immune homeostasis. Structural and functional studies using recombinant GBP4 aim to elucidate its molecular mechanisms, interactions with host/pathogen proteins, and potential as a therapeutic target or biomarker.
Overall, GBP4 recombinant protein serves as a vital tool for dissecting GBP-mediated immunity and advancing strategies to modulate infectious and inflammatory diseases.
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