| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUCA2A |
| Uniprot No | Q02747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MNAFLLSALCLLGAWAALAGGVTVQDGNFSFSLESVKKLKDLQEPQEPRVGKLRNFAPIPGEPVVPILCSNPNFPEELKPLCKEPNAQEILQRLEEIAEDPGTCEICAYAACTGC |
| 预测分子量 | 12,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GUCA2A重组蛋白的假设参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **"Recombinant Expression and Functional Analysis of Human GUCA2A in Intestinal Epithelial Cells"**
*Authors: Zhang Y, Chen L, Wang F.*
**摘要**：本研究通过哺乳动物表达系统成功制备重组GUCA2A蛋白，证实其可激活鸟苷酸环化酶C(GC-C)信号通路，并调节肠道离子转运功能。
2. **"Structural Characterization of GUCA2A Using NMR Spectroscopy"**
*Authors: Müller S, Schmidt A, Braun V.*
**摘要**：通过核磁共振(NMR)解析重组GUCA2A蛋白的三维结构，揭示其与GC-C受体结合的分子机制，为药物设计提供结构基础。
3. **"Therapeutic Efficacy of Recombinant GUCA2A in Mouse Models of Diarrhea"**
*Authors: Kim H, Tanaka M, Patel R.*
**摘要**：在腹泻小鼠模型中，重组GUCA2A蛋白显著抑制肠道液体分泌，证明其作为潜在止泻药物的应用价值。
4. **"Optimization of GUCA2A Production in E. coli for High-Yield Purification"**
*Authors: Gupta P, Kumar S, Dey S.*
**摘要**：优化大肠杆菌表达系统，实现GUCA2A重组蛋白的高效可溶性表达与纯化，并验证其生物活性。
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**建议**：如需具体文献，可通过PubMed或Google Scholar搜索关键词“GUCA2A recombinant protein”“Guanylin expression”或结合研究领域(如结构、功能、治疗应用)进一步筛选。
**Background of GUCA2A Recombinant Protein**
GUCA2A, or guanylate cyclase activator 2A, is a protein encoded by the *GUCA2A* gene in humans. It is primarily expressed in intestinal epithelial cells and serves as an endogenous activator of membrane-bound guanylate cyclase C (GC-C), a receptor critical for regulating cyclic guanosine monophosphate (cGMP)-dependent signaling pathways. GUCA2A is also known as uroguanylin, a peptide hormone that binds to GC-C to stimulate intracellular cGMP production. This signaling cascade plays a vital role in maintaining electrolyte and fluid homeostasis, particularly in the gastrointestinal tract, by regulating ion transport and water secretion.
Recombinant GUCA2A protein is engineered using biotechnological methods, often expressed in *E. coli* or mammalian cell systems, to ensure proper folding and post-translational modifications. The recombinant form mimics the native protein's structure and function, enabling researchers to study its interactions with GC-C and downstream effects in controlled experimental settings. Its production typically involves affinity chromatography and rigorous quality controls to confirm purity, stability, and bioactivity.
Research on GUCA2A recombinant protein has broad implications. It is used to investigate digestive physiology, secretory diarrhea mechanisms, and potential therapies for disorders like irritable bowel syndrome (IBS) or colorectal cancer, where GUCA2A/GC-C signaling is dysregulated. Additionally, it serves as a tool for developing diagnostic assays or targeted drugs, given its role as a biomarker in certain gastrointestinal pathologies. Studies also explore its extra-intestinal functions, including possible roles in blood pressure regulation and renal fluid balance.
Overall, GUCA2A recombinant protein provides a valuable resource for understanding cGMP-mediated cellular processes and advancing therapeutic strategies for diseases linked to GC-C pathway dysfunction.
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