| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTSR2 |
| Uniprot No | O95665 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-410aa |
| 氨基酸序列 | METSSPRPPRPSSNPGLSLDARLGVDTRLWAKVLFTALYALIWALGAAGNALSAHVVLKARAGRAGRLRHHVLSLALAGLLLLLVGVPVELYSFVWFHYPWVFGDLGCRGYYFVHELCAYATVLSVAGLSAERCLAVCQPLRARSLLTPRRTRWLVALSWAASLGLALPMAVIMGQKHELETADGEPEPASRVCTVLVSRTALQVFIQVNVLVSFVLPLALTAFLNGVTVSHLLALCSQVPSTSTPGSSTPSRLELLSEEGLLSFIVWKKTFIQGGQVSLVRHKDVRRIRSLQRSVQVLRAIVVMYVICWLPYHARRLMYCYVPDDAWTDPLYNFYHYFYMVTNTLFYVSSAVTPLLYNAVSSSFRKLFLEAVSSLCGEHHPMKRLPPKPQSPTLMDTASGFGDPPETRT |
| 预测分子量 | 45,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTSR2重组蛋白的3篇代表性文献示例(内容基于公开研究领域知识总结，非真实文献)：
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1. **文献名称**：*Structural Insights into Neurotensin Receptor 2 Activation by Cryo-EM*
**作者**：Zhang L, et al.
**摘要**：本研究通过冷冻电镜技术解析了NTSR2重组蛋白与神经降压素结合的复合物结构，揭示了其配体结合口袋的构象变化及下游信号传导机制，为靶向NTSR2的药物设计提供结构基础。
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2. **文献名称**：*Expression and Functional Characterization of Recombinant NTSR2 in Cancer Cell Lines*
**作者**：Wang J, et al.
**摘要**：报道了在HEK293细胞中高效表达NTSR2重组蛋白的方法，并验证其在肿瘤细胞中的促增殖功能，证明NTSR2可能通过MAPK通路影响癌症进展。
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3. **文献名称**：*Development of a High-Yield Purification Protocol for NTSR2 Recombinant Protein*
**作者**：Smith R, et al.
**摘要**：优化了NTSR2重组蛋白的杆状病毒表达系统及亲和层析纯化流程，获得高纯度蛋白用于体外结合实验，显著提升下游药理学研究效率。
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如需具体文献，建议通过PubMed或SciHub检索关键词“NTSR2 recombinant protein”“Neurotensin receptor 2 structure/function”获取最新研究。
**Background of NTSR2 Recombinant Protein**
Neurotensin receptor type 2 (NTSR2) is a G protein-coupled receptor (GPCR) that binds neurotensin (NT), a 13-amino-acid neuropeptide involved in diverse physiological processes, including pain modulation, thermoregulation, and inflammatory responses. Unlike NTSR1. its high-affinity counterpart, NTSR2 exhibits distinct tissue distribution, signaling pathways, and lower binding affinity for neurotensin. Both receptors are implicated in neurological disorders, cancer progression, and metabolic dysregulation, but NTSR2’s precise biological roles remain less understood due to its complex signaling and overlapping functions with NTSR1.
Recombinant NTSR2 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor material for research. Its production often involves tagging (e.g., His-tag) for isolation and stabilization in membrane-like environments (e.g., nanodiscs or detergent micelles) to maintain structural integrity. This enables detailed studies on receptor-ligand interactions, activation mechanisms, and downstream signaling cascades.
NTSR2 has gained attention as a therapeutic target, particularly in cancer, where neurotensin signaling promotes tumor growth and metastasis. Structural studies using recombinant NTSR2. coupled with cryo-EM or X-ray crystallography, aim to elucidate its activation states and guide drug design. Additionally, its role in neurodegenerative diseases (e.g., Parkinson’s) and psychiatric conditions (e.g., schizophrenia) underscores its biomedical relevance.
Despite challenges in distinguishing NTSR2-specific effects from NTSR1. recombinant protein tools continue to advance mechanistic insights, offering potential for selective therapeutics targeting neurotensin pathways. Ongoing research focuses on unraveling its physiological impact and therapeutic applications in precision medicine.
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