| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OLIG2 |
| Uniprot No | Q13516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFDSDASLVSSRPSSPEPDDLFL PARSKGSSGSAFTG GTVSSSTPSDCPPELSAELRGAMGSAGAHPGDKL GGSGFKSSSSSTSSSTSSAAASSTKKDKKQ MTEPELQQLRLKINSRER KRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTN SL EEMKRLVSEIYGGHHAGFHPSACGGLAHSAPLPAATAHPAAAAHAAHHPA VHHPILPPAAAA AAAAAAAAAVSSASLPGSGLPSVGSIRPPHGLLKSP SAAAAAPLGGGGGGSGASGGFQHWGGMP CPCSMCQVPPPHHHVSAMGA GSLPRLTSDAK |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与OLIG2重组蛋白相关的3篇文献摘要概述：
1. **文献名称**：*OLIG2 regulates purine metabolism and promotes malignant progression in glioma*
**作者**：Wang Y, et al.
**摘要**：研究利用重组OLIG2蛋白揭示其在胶质瘤细胞中的代谢调控功能，发现OLIG2通过激活嘌呤合成通路促进肿瘤侵袭，为靶向治疗提供依据。
2. **文献名称**：*Structural basis of OLIG2 phosphorylation-mediated regulation in neural stem cell differentiation*
**作者**：Li X, et al.
**摘要**：通过大肠杆菌表达系统获得重组OLIG2蛋白，结合晶体学分析发现其磷酸化修饰位点，阐明该修饰如何影响OLIG2与DNA结合能力及神经干细胞分化方向。
3. **文献名称**：*Recombinant OLIG2 protein enhances direct reprogramming of astrocytes into motor neurons*
**作者**：Smith J, et al.
**摘要**：研究证明体外纯化的OLIG2重组蛋白与小分子联用可高效将星形胶质细胞转分化为运动神经元，为脊髓损伤修复提供新策略。
注：以上文献信息为示例性概括，实际引用需根据具体研究内容匹配正式出版物。建议通过PubMed或Web of Science以“OLIG2 recombinant protein”为关键词检索最新实验论文。
**Background of OLIG2 Recombinant Protein**
OLIG2 (Oligodendrocyte Transcription Factor 2) is a basic helix-loop-helix (bHLH) transcription factor critical for central nervous system (CNS) development. It plays pivotal roles in neural progenitor cell specification, oligodendrocyte differentiation, and motor neuron development. Initially identified for its involvement in oligodendrocyte lineage commitment, OLIG2 is also essential for maintaining neural stem cells and regulating the balance between neurogenesis and gliogenesis during embryogenesis.
Recombinant OLIG2 protein is engineered *in vitro* using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. Structurally, it retains conserved domains, including the bHLH region responsible for DNA binding and protein dimerization. Its function is context-dependent, influenced by post-translational modifications and interactions with co-factors like NGN2 or SOX10.
In disease research, OLIG2 is implicated in gliomas, particularly glioblastoma, where its overexpression drives tumor proliferation and resistance to therapy. It also serves as a biomarker for certain CNS malignancies. Recombinant OLIG2 is widely used to study neural differentiation *in vitro*, model neurodegenerative diseases, and screen therapeutic agents targeting OLIG2-associated pathways.
Overall, OLIG2 recombinant protein is a vital tool for unraveling CNS development mechanisms and exploring therapeutic strategies for neurological disorders and cancers.
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