| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPS6 |
| Uniprot No | Q7L5N1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MAAAAAAAAATNGTGGSSGMEVDAAVVPSVMACGVTGSVSVALHPLVILN ISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIII DKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPL FLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGV DHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEV PFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYLGTITKTCN TMNQFVNKFNVLYDRQGIGRRMRGLFF |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COPS6重组蛋白的模拟参考文献示例，供参考。建议通过学术数据库(如PubMed、Web of Science)检索最新文献以获取真实数据：
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1. **文献名称**：*Structural and functional analysis of the COP9 signalosome subunit CSN6/Cops6*
**作者**：Wei N, Serino G, Deng XW
**摘要**：本研究利用重组表达的COPS6蛋白，结合X射线晶体学分析，揭示了其在COP9信号复合体中的结构作用，并证明其通过保守的PCI结构域参与复合体组装及底物识别。
2. **文献名称**：*Recombinant COPS6 regulates the activity of Cullin-RING ubiquitin ligases in vitro*
**作者**：Cope GA, Deshaies RJ
**摘要**：通过大肠杆菌系统表达并纯化重组COPS6蛋白，发现其能够与Cullin蛋白相互作用，调控泛素连接酶的活性，从而影响蛋白质泛素化及降解过程。
3. **文献名称**：*Expression and purification of human COPS6 for interaction proteomics*
**作者**：Schwechheimer C, et al.
**摘要**：开发了一种基于哺乳动物细胞的重组COPS6表达体系，结合质谱分析鉴定了其互作蛋白网络，强调了COPS6在细胞周期调控和DNA损伤应答中的功能。
4. **文献名称**：*Biochemical characterization of recombinant COPS6 in the deneddylation pathway*
**作者**：Lyapina S, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度COPS6重组蛋白，证明其与CSN5协同催化Cullin蛋白的去nedd化反应，维持泛素连接酶动态平衡。
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**注意**：以上为模拟文献，实际研究需参考真实发表的论文。建议结合关键词“COPS6 recombinant”“COP9 signalosome”“CSN6”等进行检索。
**Background of COPS6 Recombinant Protein**
COPS6 (COP9 signalosome subunit 6), also known as CSN6. is a critical component of the COP9 signalosome (CSN), an evolutionarily conserved protein complex involved in regulating ubiquitin-dependent protein degradation. The CSN complex, composed of eight subunits (CSN1–CSN8), plays a central role in modulating the activity of cullin-RING ubiquitin ligases (CRLs), which mediate substrate recognition in the ubiquitin-proteasome system (UPS). COPS6 contributes to maintaining the stability and functionality of the CSN complex, primarily through its interaction with other subunits and regulatory proteins.
Structurally, COPS6 contains a PCI (Proteasome, COP9. eIF3) domain, facilitating protein-protein interactions within the CSN complex. It influences deneddylation—the removal of NEDD8 (a ubiquitin-like modifier) from cullin proteins—to regulate CRL activity dynamically. Dysregulation of COPS6 has been linked to various pathologies, including cancer, neurodegenerative disorders, and immune dysfunction, underscoring its importance in cellular homeostasis.
Recombinant COPS6 protein is engineered for in vitro studies to dissect its biochemical properties, structural interactions, and mechanisms in ubiquitination pathways. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains post-translational modifications critical for its function, enabling researchers to explore its role in disease models or screen for therapeutic agents targeting CSN-associated pathways. Studies using recombinant COPS6 have advanced our understanding of its dual roles in promoting protein stability and modulating degradation, highlighting its potential as a therapeutic target in diseases with UPS dysregulation.
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