| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC7A9 |
| Uniprot No | P82251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-487aa |
| 氨基酸序列 | MGDTGLRKRREDEKSIQSQEPKTTSLQKELGLISGISIIVGTIIGSGIFV SPKSVLSNTEAVGPCLIIWAACGVLATLGALCFAELGTMITKSGGEYPYL MEAYGPIPAYLFSWASLIVIKPTSFAIICLSFSEYVCAPFYVGCKPPQIV VKCLAAAAILFISTVNSLSVRLGSYVQNIFTAAKLVIVAIIIISGLVLLA QGNTKNFDNSFEGAQLSVGAISLAFYNGLWAYDGWNQLNYITEELRNPYR NLPLAIIIGIPLVTACYILMNVSYFTVMTATELLQSQAVAVTFGDRVLYP ASWIVPLFVAFSTIGAANGTCFTAGRLIYVAGREGHMLKVLSYISVRRLT PAPAIIFYGIIATIYIIPGDINSLVNYFSFAAWLFYGLTILGLIVMRFTR KELERPIKVPVVIPVLMTLISVFLVLAPIISKPTWEYLYCVLFILSGLLF YFLFVHYKFGWAQKISKPITMHLQMLMEVVPPEEDPE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC7A9重组蛋白的3篇参考文献的简要总结：
1. **"Functional characterization of recombinant human SLC7A9 cystinuria-associated transporter"**
- **作者**: Font-Llitjós M, et al.
- **摘要**: 该研究通过在大肠杆菌和哺乳动物细胞中重组表达SLC7A9蛋白，结合体外转运实验，证实其与SLC3A1形成异源二聚体，并证明其在胱氨酸和二碱基氨基酸跨膜运输中的关键作用。突变分析揭示了与胱氨酸尿症相关的致病机制。
2. **"Structural insights into the transport mechanism of SLC7A9 in complex with SLC3A1"**
- **作者**: Lee J, et al.
- **摘要**: 通过冷冻电镜技术解析了SLC7A9与SLC3A1复合体的三维结构，阐明了其底物结合位点和跨膜转运的构象变化，为理解胱氨酸尿症的分子病理及靶向药物设计提供了结构基础。
3. **"Expression and purification of recombinant SLC7A9 for biochemical analysis"**
- **作者**: Zhang Y, et al.
- **摘要**: 开发了一种基于昆虫细胞表达系统的SLC7A9重组蛋白高效纯化方案，并通过表面等离子体共振(SPR)技术验证了其与胱氨酸的结合活性，为后续功能研究提供了可靠蛋白来源。
注：以上文献为示例性内容，实际引用时需根据具体论文调整。建议通过PubMed或Google Scholar检索关键词“SLC7A9 recombinant”获取最新研究。
**Background of SLC7A9 Recombinant Protein**
SLC7A9. a member of the solute carrier family 7 (SLC7), encodes a subunit of the heterodimeric amino acid transporter known as b⁰,+AT. This protein plays a critical role in the reabsorption of cationic and neutral amino acids, as well as cystine, across renal and intestinal epithelial cells. SLC7A9 partners with SLC3A1 (rBAT) via a disulfide bond to form the functional transporter complex, which localizes to the apical membrane of proximal renal tubules and small intestinal cells. This complex is essential for maintaining amino acid homeostasis and preventing urinary loss of dibasic amino acids and cystine.
Mutations in the *SLC7A9* gene are linked to autosomal recessive cystinuria, a hereditary disorder characterized by impaired renal reabsorption of cystine and dibasic amino acids. This leads to cystine stone formation in the urinary tract, causing recurrent nephrolithiasis and potential kidney damage. Studying SLC7A9’s structure-function relationships and pathogenic variants is vital for understanding disease mechanisms and developing targeted therapies.
Recombinant SLC7A9 protein is produced using expression systems such as bacterial (e.g., *E. coli*) or mammalian cells (e.g., HEK293), enabling in vitro analysis of its transport activity, substrate specificity, and interaction with SLC3A1. Purification techniques like affinity chromatography yield high-purity protein for functional assays, structural studies (e.g., cryo-EM), or drug screening. Recombinant SLC7A9 also aids in exploring the impact of mutations on transporter stability, trafficking, or ligand binding, providing insights into genotype-phenotype correlations in cystinuria.
Overall, SLC7A9 recombinant protein serves as a key tool for advancing research on amino acid transport biology, genetic disorders, and therapeutic strategies targeting transporter dysfunction.
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