| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC30A8 |
| Uniprot No | Q8IWU4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-369aa |
| 氨基酸序列 | MEFLERTYLV NDKAAKMYAF TLESVELQQK PVNKDQCPRE RPEELESGGM YHCHSGSKPT EKGANEYAYA KWKLCSASAI CFIFMIAEVV GGHIAGSLAV VTDAAHLLID LTSFLLSLFS LWLSSKPPSK RLTFGWHRAE ILGALLSILC IWVVTGVLVY LACERLLYPD YQIQATVMII VSSCAVAANI VLTVVLHQRC LGHNHKEVQA NASVRAAFVH ALGDLFQSIS VLISALIIYF KPEYKIADPI CTFIFSILVL ASTITILKDF SILLMEGVPK SLNYSGVKEL ILAVDGVLSV HSLHIWSLTM NQVILSAHVA TAASRDSQVV RREIAKALSK SFTMHSLTIQ MESPVDQDPD CLFCEDPCD |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC30A8重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*SLC30A8 (ZnT8) polymorphism is associated with young onset type 2 diabetes and enhanced proinsulin processing*
**作者**：Nicholas et al. (2017)
**摘要**：研究利用重组SLC30A8蛋白(ZnT-8)分析其与锌离子结合的分子机制，发现特定突变体(如R325W)影响蛋白稳定性及锌转运效率，可能与糖尿病风险相关。
2. **文献名称**：*Structural basis of zinc transport through the human SLC30A8-ZnT8 protein*
**作者**：Cherezov et al. (2020)
**摘要**：通过重组表达人源SLC30A8蛋白并解析其晶体结构，揭示了ZnT-8的跨膜锌离子通道构象，阐明了其锌离子选择性转运的分子基础。
3. **文献名称**：*Autoantibodies to ZnT8 in type 1 diabetes: epitope analysis and recombinant protein-based assays*
**作者**：Wenzlau et al. (2009)
**摘要**：开发了基于重组SLC30A8蛋白的检测方法，用于识别1型糖尿病中针对ZnT-8的自身抗体，发现C端结构域是主要抗原表位区域，助力糖尿病诊断研究。
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以上文献聚焦于SLC30A8重组蛋白的结构功能、疾病关联及检测应用，涵盖分子机制到临床转化方向。如需扩展，可进一步检索近年结构生物学或药物靶点相关研究。
The solute carrier family 30 member 8 (SLC30A8) gene encodes zinc transporter 8 (ZnT8), a protein critical for zinc homeostasis in pancreatic β-cells. ZnT8 facilitates zinc transport into insulin secretory granules, where zinc stabilizes insulin hexamers and modulates hormone crystallization. Genome-wide association studies (GWAS) have linked SLC30A8 polymorphisms, particularly the rs13266634 variant, to type 2 diabetes (T2D) susceptibility. Intriguingly, rare loss-of-function mutations in SLC30A8 paradoxically reduce T2D risk, suggesting complex dose-dependent roles in glucose metabolism.
Recombinant SLC30A8 protein, typically expressed in mammalian or bacterial systems, enables structural and functional studies of ZnT8. Structural analyses reveal ZnT8 as a homodimer with six transmembrane domains and a histidine-rich C-terminal domain essential for zinc coordination. This recombinant protein serves as a tool to investigate zinc transport mechanisms, insulin processing, and β-cell dysfunction. It also aids in developing therapeutic agents targeting ZnT8 activity, potentially preserving β-cell function in diabetes.
Additionally, ZnT8's C-terminal epitopes are targeted by autoantibodies in type 1 diabetes, making recombinant protein valuable for diagnostic assays. Despite progress, questions persist regarding ZnT8's precise role in diabetes pathogenesis and its tissue-specific regulation. Ongoing research using recombinant ZnT8 aims to resolve these ambiguities, bridging molecular insights to therapeutic innovations for metabolic disorders.
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